LOCUS       sequence-111803-        1487 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Burkel BM, von Dassow G, Bement WM
  TITLE     Versatile fluorescent probes for actin filaments based on the 
            actin-binding domain of utrophin.
  JOURNAL   Cell Motil Cytoskeleton. 2007 Nov . 64(11):822-32.
  PUBMED    17685442
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Motil 
            Cytoskeleton. 2007 Nov . 64(11):822-32."
COMMENT     Sequence Label: mRFP-UtrCH
FEATURES             Location/Qualifiers
     source          1..1487
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             12..686
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             12..686
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(142..160)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     410..429
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     604..627
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 ggatccaaac catggcctcc tccgaggacg tcatcaagga gttcatgcgc ttcaaggtgc
       61 gcatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
      121 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg
      181 cctgggacat cctgtcccct cagttccagt acggctccaa ggcctacgtg aagcaccccg
      241 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
      301 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg
      361 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc
      421 agaagaagac catgggctgg gaggcctcca ccgagcggat gtaccccgag gacggcgccc
      481 tgaagggcga gatcaagatg aggctgaagc tgaaggacgg cggccactac gacgccgagg
      541 tcaagaccac ctacatggcc aagaagcccg tgcagctgcc cggcgcctac aagaccgaca
      601 tcaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgcg
      661 ccgagggccg ccactccacc ggcgcctccg gaaccatggc caagtatgga gaacatgaag
      721 ccagtcctga caatgggcag aacgaattca gtgatatcat taagtccaga tctgatgaac
      781 acaatgacgt acagaagaaa acctttacca aatggataaa tgctcgattt tcaaagagtg
      841 ggaaaccacc catcaatgat atgttcacag acctcaaaga tggaaggaag ctattggatc
      901 ttctagaagg cctcacagga acatcactgc caaaggaacg tggttccaca agggtacatg
      961 ccttaaataa cgtcaacaga gtgctgcagg ttttacatca gaacaatgtg gaattagtga
     1021 atataggggg aaccgacatt gtggatggaa atcacaaact gactttgggg ttactttgga
     1081 gcatcatttt gcactggcag gtgaaagatg tcatgaagga tgtcatgtcg gacctgcagc
     1141 agacgaacag tgagaagatc ctgctcagct gggtgcgtca gaccaccagg ccctacagcc
     1201 aagtcaacgt cctcaacttc accaccagct ggacagatgg actcgccttt aatgctgtcc
     1261 tccaccgaca taaacctgat ctcttcagct gggataaagt tgtcaaaatg tcaccaattg
     1321 agagacttga acatgccttc agcaaggctc aaacttattt gggaattgaa aagctgttag
     1381 atcctgaaga tgttgccgtt cggcttcctg acaagaaatc cataattatg tatttaacat
     1441 ctttgtttga ggtgctacct cagcaagtca ccatagacta actcgag
//
