LOCUS       sequence-111904-         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A human codon-optimized Cas9 for expression in S. cerevisiae 
            (budding yeast) from the TEF1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Dicarlo JE, Norville JE, Mali P, Rios X, Aach J, Church GM.
  TITLE     Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas 
            systems.
  JOURNAL   Nucleic Acids Res
  PUBMED    23460208
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res"
COMMENT     Sequence Label: p414-TEF1p-Cas9-CYC1t
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..70
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     54..70
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tttttttact tcttgctcat tagaaagaaa gcatagcaat ctaatctaag ttttctagaa
       61 ctagtggatc ccccgggaaa aatggacaag aagtactcca ttgggctcga tatcggcaca
      121 aacagcgtcg gttgggccgt cattacggac gagtacaagg tgccgagcaa aaaattcaaa
      181 gttctgggca ataccgatcg ccacagcata aagaagaacc tcattggcgc cctcctgttc
      241 gactccgggg agacggccga agccacgcgg ctcaaaagaa cagcacggcg cagatatacc
      301 cgcagaaaga atcggatctg ctacctgcag gagatcttta gtaatgagat ggctaaggtg
      361 gatgactctt tcttccatag gctggaggag tcctttttgg tggaggagga taaaaagcac
      421 gagcgccacc caatctttgg caatatcgtg gacgaggtgg cgtaccatga aaagtaccca
      481 accatatatc atctgaggaa gaagcttgta gacagtactg ataaggctga cttgcggttg
      541 atctatctcg cgctggcgca tatgatcaaa tttcggggac acttcctcat cgagggggac
      601 ctgaacccag acaacagcga tgtcgacaaa ctctttatcc aactggttca gacttacaat
      661 cagcttttcg aagagaaccc gatcaacgca tccggagttg acgccaaagc aatcctgagc
      721 gctaggctgt ccaaatcccg gcggctcgaa aacctcatcg cacagctccc tggggagaag
      781 aagaac
//
