LOCUS       sequence-112126-        6804 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Receptor integration for two-strain system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6804)
  AUTHORS   Chen Y, Kim JK, Hirning AJ, Josic K, Bennett MR
  TITLE     SYNTHETIC BIOLOGY. Emergent genetic oscillations in a synthetic 
            microbial consortium.
  JOURNAL   Science. 2015 Aug 28;349(6251):986-9. doi: 10.1126/science.aaa3794.
  PUBMED    26315440
REFERENCE   2  (bases 1 to 6804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2015-08-28- 28"; volume: "349"; 
            issue: "6251"; pages: "986-9"
COMMENT     Sequence Label: pCH119
FEATURES             Location/Qualifiers
     source          1..6804
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..34
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     terminator      98..125
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(735..1394)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1309..1328
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1395..1497)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    1554..1587
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     terminator      complement(1724..1751)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        complement(join(2713..2719,2720..2736,2737..2742))
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     CDS             complement(2871..2900)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(3091..3113)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3102..3118
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        join(3186..3191,3192..3208,3209..3215)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    4110..4341
                     /gene="attP"
                     /label=lambda attP
                     /bound_moiety="lambda integrase"
                     /note="integrase from phage lambda"
     terminator      4376..4462
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(5000..5950)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      5998..6220
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(6722..6756)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gaagttccta ttctctagaa agtataggaa cttcaacgcc atgagcggcc tcatttctta
       61 ttctgagtta caacagtccg caccgctgtc tcacactggc tcaccttcgg gtgggccttt
      121 ctgcgtttat atactagaga gagaatataa aaagccagat tattaatccg gcttttttat
      181 tatttcctag cggccgcagg cggtagctcc ttccggtggg cgcggggcat gactatcgtc
      241 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgccc
      301 aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gccctgcacc
      361 attatgttcc ggatctgcat cgcaggatgc tgctggctac cctgtggaac acctacatct
      421 gtattaacga agcgctaacc gtttttatca ggctctggga ggcagaataa atgatcatat
      481 cgtcaattat tacctccacg gggagagcct gagcaaactg gcctcaggca tttgagaagc
      541 acacggtcac actgcttccg gtagtcaata aaccggtaaa ccagcaatag acataagcgg
      601 ctatttaacg accctgccct gaaccgacga ccgggtcgaa tttgctttcg aatttctgcc
      661 attcatccgc ttattatcac ttattcaggc gtagcaccag gcgtttaagg gcaccaataa
      721 ctgccttaaa aaaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta
      781 agcattctgc cgacatggaa gccatcacag acggcatgat gaacctgaat cgccagcggc
      841 atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag
      901 ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag
      961 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac
     1021 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag
     1081 agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc
     1141 catatcacca gctcaccgtc tttcattgcc atacggaatt ccggatgagc attcatcagg
     1201 cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt
     1261 aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga
     1321 aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg
     1381 atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg
     1441 cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg ccgatcaacg
     1501 tctcattttc gccaaaagtt ggcccagggc ttcccggtat caacagggac accgaagttc
     1561 ctattctcta gaaagtatag gaacttctgc tcatgtttga cagcttatca tcgatgcata
     1621 atgtgcctgt caaatggacg aagcagggat tctgctagct aggaaataat aaaaaagccg
     1681 gattaataat ctggcttttt atattctctc tctagtatat aaacgcagaa aggcccaccc
     1741 gaaggtgagc cagtgtgaga cagcggtgcg gactgttgta actcagaata agaaatgagg
     1801 ccgctcatgg cgttgactct cagtcatagt atcgtggtat caccggttgg ttccactctc
     1861 tgttgcgggc aacttcagca gcacgtaggg gaagcttatt agatgagacc cagcgccgcg
     1921 gcgtaggcgg cagccagcgt cttgttcggc gcgtcgaact tcttctggat gttcttgtgg
     1981 tggaagttca ccgtgctctc ggagatgctc aggatgatgg cgatttcccc ggaactcttg
     2041 ccgtcggcgg tccattgcag gatctcgcgt tcgcgatggc tcaggcagac cgggttggac
     2101 atcagcatcg gatgctccag gtcggtcagc ttctgggtca gcaactcgat catgcaacgc
     2161 agccgcaggc ggatttcctc gcgctcgaag ctggagatgt tctgctggtc gcgcgccacg
     2221 gaaagcacgc tgagcaaatt gttcggcgcg cggatcggca aggtcgcgcc gacacagagg
     2281 ccccaatcgc gagcctcgtt ccagagcatc cggctctggt cgaacaggct gtcgctccag
     2341 accaccattt ccgaggagcg caggccgttg aggatcgccg gatccacggc cccgtagttc
     2401 tgcatctggt atcgctccag ccaggccttg ggataggtgc catggacctc ggtcttcggc
     2461 cgggtgaagg gaatcgtgtg gcgcacgcca taggcgtagt aatcgaagcc caggcgccgc
     2521 acttcctttt ccaggacggc gaacacgccc tggctgtcgt ggatcggctg catctcgcta
     2581 cgcaaaccgt cccaccacag caaaaagcct ccgtcattcc tcatactaga atttctcctt
     2641 agcatgatta agatgtttca gtacgaaaat tgctttcatt gttgatctcc tttttaagtg
     2701 gcatgctcca cacattatac gagccggatg attaattgtc aatcgacagt tcataggtga
     2761 ttgctcagga catttctgtt agaaggaatc gttttcctta cttttcctta cgcacaagag
     2821 ttccgtagct gttcaagttt gtgtttcaac tgttctcgtc gtttccgcaa caagtcctct
     2881 tcagaaatga gcttttgctc ctctgcttgg acggacagga tgtatgctgt ggctttttta
     2941 aggataacta ccttgggggc cttttcattg ttttccaact ccgggatctg gtcacgcagg
     3001 gcaaaaaagc tccgttttag ctcgttcctc ctctggcgct ccaagacgtt gtgtgttcgc
     3061 ctcttgacat tctcctcggt gtccgagggc cctgtgtgaa attgttatcc gctcacaatt
     3121 ccacacagac gtcggaattg ccagctgggg cgccctctgg taaggttggg aagctcgagc
     3181 acgagttgac aattaatcat ccggctcgta taatgtgtgg agcatgccac ttaaaaagga
     3241 gatcaacaat gaaagcaatt ttcgtactga aacatcttaa tcatgctaag gagaaattct
     3301 agtatgattg agaataccta tagcgaaaag ttcgagtccg cgttcgaaca gatcaaggcg
     3361 gcggccaacg tggatgccgc catccgtatt ctccaggcgg aatataacct cgatttcgtc
     3421 acctaccatc tcgcccagac gatcgcgagc aagatcgatc cgcccttcgt gcgcaccacc
     3481 tatccggatg cctgggtttc ccgctacctc ctcaacagct atgtgaaggt cgatccgatc
     3541 gtcaagcagg gcttcgaacg ccagctgccc ttcgactgga gcgaggtcga accgacgccg
     3601 gaggcctatg ccatgctggt cgacgcccag aaacacggca tcggtggcaa tggctactcc
     3661 atccccgtcg ccgacaaggc gcagcgccgc gccctgctgt cgctgaatgc ccgtataccg
     3721 gccgacgaat ggaccgagct tgtgcgccgc tgccgcaacg agtggatcga gatcgcccat
     3781 ctgatccacc gcaaggccgt ctatgagctg catggcgaaa acgatccggt gccggcattg
     3841 tcgccgcgcg agatcgagtg tctgcactgg accgccctcg gcaaggatta caaggatatt
     3901 tcggtcatcc tgggcatatc agagcatacc acacgcgatt acctgaagac cgcccgcttc
     3961 aagctcggct gcgccacgat ctcggccgcc gcgtcgcggg ctgttcaatt gcgcatcatc
     4021 aatccctgag agctcctaga gtgcgcgaca ggtttgatga caaaaaatta gcgcaagaag
     4081 acaaaaatca ccttgcgcta atgctctgtt acaggtcact aataccatct aagtagttga
     4141 ttcatagtga ctgcatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa
     4201 tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct tttttatact
     4261 aagttggcat tataaaaaag cattgcttat caatttgttg caacgaacag gtcactatca
     4321 gtcaaaataa aatcattatt tgatttcaat tttgtcccac tccccctaga ggcatcaaat
     4381 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     4441 cgctctcctg agtaggacaa atccgccgcc ctagacctag ggtacgggtt ttgctgcccg
     4501 caaacgggct gttctggtgt tgctagtttg ttatcagaat cgcagatccg gcttcagccg
     4561 gtttgccggc tgaaagcgct atttcttcca gaattgccat gattttttcc ccacgggagg
     4621 cgtcactggc tcccgtgttg tcggcagctt tgattcgata agcagcatcg cctgtttcag
     4681 gctgtctatg tgtgactgtt gagctgtaac aagttgtctc aggtgttcaa tttcatgttc
     4741 tagttgcttt gttttactgg tttcacctgt tctattaggt gttacatgct gttcatctgt
     4801 tacattgtcg atctgttcat ggtgaacagc tttaaatgca ccaaaaactc gtaaaagctc
     4861 tgatgtatct atctttttta caccgttttc atctgtgcat atggacagtt ttccctttga
     4921 tatctaacgg tgaacagttg ttctactttt gtttgttagt cttgatgctt cactgataga
     4981 tacaagagcc ataagaacct cagatccttc cgtatttagc cagtatgttc tctagtgtgg
     5041 ttcgttgttt ttgcgtgagc catgagaacg aaccattgag atcatgctta ctttgcatgt
     5101 cactcaaaaa ttttgcctca aaactggtga gctgaatttt tgcagttaaa gcatcgtgta
     5161 gtgtttttct tagtccgtta cgtaggtagg aatctgatgt aatggttgtt ggtattttgt
     5221 caccattcat ttttatctgg ttgttctcaa gttcggttac gagatccatt tgtctatcta
     5281 gttcaacttg gaaaatcaac gtatcagtcg ggcggcctcg cttatcaacc accaatttca
     5341 tattgctgta agtgtttaaa tctttactta ttggtttcaa aacccattgg ttaagccttt
     5401 taaactcatg gtagttattt tcaagcatta acatgaactt aaattcatca aggctaatct
     5461 ctatatttgc cttgtgagtt ttcttttgtg ttagttcttt taataaccac tcataaatcc
     5521 tcatagagta tttgttttca aaagacttaa catgttccag attatatttt atgaattttt
     5581 ttaactggaa aagataaggc aatatctctt cactaaaaac taattctaat ttttcgcttg
     5641 agaacttggc atagtttgtc cactggaaaa tctcaaagcc tttaaccaaa ggattcctga
     5701 tttccacagt tctcgtcatc agctctctgg ttgctttagc taatacacca taagcatttt
     5761 ccctactgat gttcatcatc tgagcgtatt ggttataagt gaacgatacc gtccgttctt
     5821 tccttgtagg gttttcaatc gtggggttga gtagtgccac acagcataaa attagcttgg
     5881 tttcatgctc cgttaagtca tagcgactaa tcgctagttc atttgctttg aaaacaacta
     5941 attcagacat acatctcaat tggtctaggt gattttaatc actataccaa ttgagatggg
     6001 ctagtcaatg ataattacta gtccttttcc tttgagttgt gggtatctgt aaattctgct
     6061 agacctttgc tggaaaactt gtaaattctg ctagaccctc tgtaaattcc gctagacctt
     6121 tgtgtgtttt ttttgtttat attcaagtgg ttataattta tagaataaag aaagaataaa
     6181 aaaagataaa aagaatagat cccagccctg tgtataactc actactttag tcagttccgc
     6241 agtattacaa aaggatgtcg caaacgctgt ttgctcctct acaaaacaga ccttaaaacc
     6301 ctaaaggctt aagtagcacc ctcgcaagct cgggcaaatc gctgaatatt ccttttgtct
     6361 ccgaccatca ggcacctgag tcgctgtctt tttcgtgaca ttcagttcgc tgcgctcacg
     6421 gctctggcag tgaatggggg taaatggcac tacaggcgcc ttttatggat tcatgcaagg
     6481 aaactaccca taatacaaga aaagcccgtc acgggcttct cagggcgttt tatggcgggt
     6541 ctgctatgtg gtgctatctg actttttgct gttcagcagt tcctgccctc tgattttcca
     6601 gtctgaccac ttcggattat cccgtgacag gtcattcaga ctggctaatg cacccagtaa
     6661 ggcagcggta tcatcaacag gcttacccgt cttactgtcc ctagtgcttg gattctcacc
     6721 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt
     6781 cattactgga tctatcaaca ggag
//
