LOCUS       sequence-112142-         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lim Lab plasmid: AmpR + PCon::glgC::gfp::T1T2 terminator + ColE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Sagawa S, Shin JE, Hussein R, Lim HN
  TITLE     Paradoxical suppression of small RNA activity at high Hfq 
            concentrations due to random-order binding.
  JOURNAL   Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
  PUBMED    26261213
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Aug 10. pii: gkv777."
COMMENT     Sequence Label: pHL671
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(78..172)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      complement(206..300)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 ttgatctttt ctcggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
       61 tcatgactag tgcttggatt ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg
      121 aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag
      181 ctctcgaacg acgtctagtg cttggattct caccaataaa aaacgcccgg cggcaaccga
      241 gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc
      301 caagcgagct cgtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
      361 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
      421 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
      481 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
      541 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
      601 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
      661 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
      721 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
      781 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
      841 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
      901 tgagaat
//
