LOCUS       sequence-112152-         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lim Lab plasmid: AmpR + PconNoHindM8::micC::Asp terminator + 
            PLlacO-1::T710::hfq::T1 terminator + PLtetO-1m9::ompC::gfp::T1T2 
            terminator + ColE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Sagawa S, Shin JE, Hussein R, Lim HN
  TITLE     Paradoxical suppression of small RNA activity at high Hfq 
            concentrations due to random-order binding.
  JOURNAL   Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
  PUBMED    26261213
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Aug 10. pii: gkv777."
COMMENT     Sequence Label: pHL2107
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(280..307)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(399..485)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     638..655
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 gtctccctgt acgattaaag cttctggata aggattatcc aattctaaaa aaaaagcccg
       61 gacgactgtt cgggcttgtc tttttatatg ttggaaaatc agtggcaatg caatggccca
      121 acagaaaata aaatctgtga caataaaggc atataacccg gggaatccac cataaccgcc
      181 aagcataaaa atgtaaacaa cgacggtgct cgaggatcct gagcggatac atatttgaat
      241 gtatttagaa aaataaacaa aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg
      301 gccttctgct taatttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg
      361 ggccgttgct tcgcaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg
      421 ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt
      481 atttgatgcc tggcagttcc ctactctcgc atggggagac cccacactac catcggcgct
      541 acggcgtttc acttctgagt tcggcatggg gtcaggtggg accaccgcgc tactgccgcc
      601 aggcaaattc tgttttatca gaccgcttct gcgttctgat ttaatctgta tcaggctgaa
      661 aatcttctct catccgccaa aacagccaag ctagctttta tttgtagagc tcatccatgc
      721 catgtgtaat cccagcagct gttacaa
//
