LOCUS       sequence-112162-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lim Lab plasmid: AmpR + PCon::glgC::gfp::T1T2 terminator + ColE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Sagawa S, Shin JE, Hussein R, Lim HN
  TITLE     Paradoxical suppression of small RNA activity at high Hfq 
            concentrations due to random-order binding.
  JOURNAL   Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
  PUBMED    26261213
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Aug 10. pii: gkv777."
COMMENT     Sequence Label: pHL671
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        171..275
                     /gene="bla"
                     /label=AmpR promoter
     CDS             460..838
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(494..513)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cgcatgctaa ccatgactaa ctcctttttt atcatctctg gaacacacac aatccgtgtg
       61 caggtccctg ccagagtcga cctgtgtgga atccattata accgccaagc ttaaaaatgt
      121 caacaacgac ggtgctcgag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
      181 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
      241 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
      301 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
      361 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
      421 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
      481 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
      541 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
      601 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
      661 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
      721 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
      781 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
      841 gcaaactatt aactggcgaa ctacttactc tagcttcccg
//
