LOCUS       sequence-112221-         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of LC3 fused to EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Lee IH, Cao L, Mostoslavsky R, Lombard DB, Liu J, Bruns NE, Tsokos 
            M, Alt FW, Finkel T
  TITLE     A role for the NAD-dependent deacetylase Sirt1 in the regulation of 
            autophagy.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Mar 4. 105(9):3374-9.
  PUBMED    18296641
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Mar 4. 105(9):3374-9."
COMMENT     Sequence Label: pEGFP-LC3 (human)
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    127..248
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(138..157)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     192..211
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(380..754)
                     /codon_start=1
                     /gene="MAP1LC3B"
                     /product="human LC3 protein"
                     /label=LC3B
                     /note="Atg8 family member found on autophagosomes"
                     /translation="MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLP
                     VLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKD
                     EDGFLYMVYASQETFGMKLSV"
     primer_bind     complement(842..863)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
       61 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
      121 acgcgttaag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
      181 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
      241 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
      301 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcagttat
      361 ctagatccgg tggatcctgc actgacaatt tcatcccgaa cgtctcctgg gaggcataga
      421 ccatgtacag gaatccatct tcatctttct cactctcata cacctctgag attggtgtgg
      481 agacgctgac catgctgtgt ccgttcacca acaggaagaa ggcctgatta gcattgagct
      541 gtaagcgcct tctaattatc ttgatgagct cactcatgtt gacatggtca ggtacaagga
      601 actttgtttt atccagaaca ggaagctgct tctcaccctt gtatcgttct attatcaccg
      661 ggattttggt tggatgctgc tctcgaataa gtcggacatc ttctactctt tgttcgaagg
      721 tgcggcgctg cttgaaggtc ttctccgacg gcatggtcgg gaattcgaag cttgagctcg
      781 agatctgagt ccggccggac ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg
      841 tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg
      901 actgggtgct caggtagtgg ttgtcgggca gcagcacggg gccgtc
//
