LOCUS       sequence-112279-        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  harbours marker genes LEU2 and LYS2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Mulleder M, Campbell K, Matsarskaia O, Eckerstorfer F, Ralser M
  TITLE     Saccharomyces cerevisiae single-copy plasmids for auxotrophy 
            compensation, multiple marker selection, and for designing 
            metabolically cooperating communities.
  JOURNAL   F1000Res. 2016 Sep 20;5:2351. eCollection 2016.
  PUBMED    27830062
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "F1000Res.";
            date: "2016-09-20"; volume: "5"; pages: "2351. eCollection 2016"
COMMENT     Sequence Label: pLK
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(21..43)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        157..396
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
ORIGIN
        1 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
       61 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggc
      121 gcgtcgagga aaactcttca atagttttgc cagcggaatt ccacttgcaa ttacataaaa
      181 aattccggcg gtttttcgcg tgtgactcaa tgtcgaaata cctgcctaat gaacatgaac
      241 atcgcccaaa tgtatttgaa gacccgctgg gagaagttca agatatataa gtaacaagca
      301 gccaatagta taaaaaaaaa tctgagttta ttacctttcc tggaatttca gtgaaaaact
      361 gctaattata gagagatatc acagagttac tcactaatga ctaacgaaaa ggtctggata
      421 gagaagttgg ataatccaac tctttcagtg ttaccacatg actttttacg cccacaacaa
      481 gaaccttata cgaaacaagc tacatattcg ttacagctac ctcagctcga tgtgcctcat
      541 gatagttttt ctaacaaata cgctgtcgct ttgagtgtat gggctgcatt gatatataga
      601 gtaaccggtg acgatgatat tgttctttat attgcgaata acaaaatctt aagattcaat
      661 attcaaccaa cgtggtcatt taatgagctg tattctacaa ttaacaatga gttgaacaag
      721 ctcaattcta ttgaggccaa tttttccttt gacgagctag ctgaaaaaat tcaaagttgc
      781 caagatctgg aaaggacccc tcagttgttc cgtttggcct ttttggaaaa ccaagatttc
      841 aaattagacg agttcaagca tcatttagtg gactttgctt tgaatttgga taccagtaat
      901 aatgcgcatg ttttgaactt aatttataac agcttactgt attcgaatga aagagtaacc
      961 attgttgcgg accaatttac tcaatatttg actgctgcgc taagcgatcc atccaattgc
     1021 ataactaaaa tctctctgat caccgcatca tccaaggata gttta
//
