LOCUS       sequence-112300-         560 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  cDNA of Akt1 with point mutation from Trp to Ala at position 80. 
            This mutation confers resistance to the MK-2206 drug.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Kajno E, McGraw TE, Gonzalez E
  TITLE     Development of a new model system to dissect isoform specific Akt 
            signaling in adipocytes.
  JOURNAL   Biochem J. 2015 Apr 9.
  PUBMED    25856301
REFERENCE   2  (bases 1 to 560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem J. 
            2015 Apr 9."
COMMENT     Sequence Label: pLVX-IRES-tdTomato-FlagAkt1W80A
FEATURES             Location/Qualifiers
     source          1..560
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     349..366
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 tcggggggcc tagacgtttt ttaacctcga ctaaacacat gctaaagcat gtgcaccgag
       61 gccccagatc agatcccata caatggggta ccttctgggc atccttcagc cccttgttga
      121 atacgcttga ggtgagccat ttgactcttt ccacaactat ccaactcaca acgtggcact
      181 ggggttgtgc cgcctttgca ggtgtatctt atacacgtgg cttttggccg cagaggcacc
      241 tgtcgccagg tggggggttc cgctgcctgc aaagggtcgc tacagacgtt gtttgtcttc
      301 aagaagcttc cagaggaact gcttccttca cgacattcaa cagaccttgc attcctttgg
      361 cgagagggga aagaccccta ggaatgctcg tcaagaagac agggccaggt ttccgggccc
      421 tcacattgcc aaaagacggc aatatggtgg aaaataacat atagacaaac gcacaccggc
      481 cttattccaa gcggcttcgg ccagtaacgt tagggggggg ggagggagag gggcgggatc
      541 ctcaggctgt gccactggct
//
