LOCUS       sequence-112305-        1103 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A lentiviral vector for the expression of mouse Nkx6.2 under 
            doxycyline control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Najm FJ, Lager AM, Zaremba A, Wyatt K, Caprariello AV, Factor DC, 
            Karl RT, Maeda T, Miller RH, Tesar PJ
  TITLE     Transcription factor-mediated reprogramming of fibroblasts to 
            expandable, myelinogenic oligodendrocyte progenitor cells.
  JOURNAL   Nat Biotechnol. 2013 May;31(5):426-33. doi: 10.1038/nbt.2561. Epub 
            2013 Apr 14.
  PUBMED    23584611
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.2561"; 
            journalName: "Nat Biotechnol"; date: "2013-05"; volume: "31"; issue:
            "5"; pages: "426-33"
COMMENT     Sequence Label: pLVX-Tight-Puro_Nkx6.2
FEATURES             Location/Qualifiers
     source          1..1103
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(854..878)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(875..913)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    925..943
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    961..979
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    996..1014
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1032..1050
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1068..1086
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 ctacaaggcg tcccccgcgc tgccaccaca cgggctaacg agcgccaagt tcgagggttt
       61 gtgctttttg agaagccgcg tgatcttctc gtcatcggag ttggggtcca ggggccggtt
      121 gtattcgtca tcgtcctccg cgtctgagcc acccaccttc agcttctcgg catccgagtc
      181 ttgcttcttt ttagccgacg ccatctctgc cgcgtgccgc ttgcgccact tggtccgccg
      241 attctggaac cacaccttca cttggctctc ggtcatgccc agagagtagg caagccgcgc
      301 gcgctctggg cctgccaaat acttggtctg ctcgaaagtc ttctccagcg cgaagatctg
      361 ctggccggag aaagtcggcc gcgagtgttt cttcttgcca tccttatcca ggaccccgcc
      421 ggcttgggcg gagccggcca gtcgcgggtc cctccaggga gagccctgca ccaccccagg
      481 ccagaagatg ggcgggcgcc caggcagttc cgccagcggc ttggggtagc cccgagccac
      541 ggcggctgcg ggcccgaagt agacacctgc agacgaggcg agcccgttga gacggggcag
      601 acttcctagg aggccgccac ccgctgcgcc caccggccgt cccaggatgt cgctgatgcc
      661 gtgcggagtg cctagaggca actgcgcgcc aaggctgcct agggcgggtg tcttgaagcc
      721 cgccgggccc tgcagcgcgt aggggaacag cgacgtcttc atctcagcca tgttgtgcag
      781 cgcggctaaa ggcgcgctgc tcagcacaaa cgcacccggg cggttagcgt ccatggtgga
      841 tccttctcca ggcgatctga cggttcacta aacgagctct gcttatatag gcctcccacc
      901 gtacacgcct acctcgacat acgttctcta tcactgatag ggagtaaact cgacatacgt
      961 tctctatcac tgatagggat aaactcgaca tacgttctct atcactgata gggagtaaac
     1021 tcgacatacg ttctctatca ctgataggga gtaaactcga catacgttct ctatcactga
     1081 tagggagtaa actcgacatc gtt
//
