LOCUS       sequence-112393-         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expression of human codon-optimized Staphylococcus aureus Cas9 
            nuclease and GFP, plasmid optimized for expression in human 
            pluripotent stem cells and other mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  TITLE     Staphylococcus aureus Cas9
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSaCas9_GFP
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             27..92
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             99..119
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gcaaagaatt cgcggccgcc accatggact ataaggacca cgacggagac tacaaggatc
       61 atgatattga ttacaaagac gatgacgata agatggcccc gaagaaaaag cgcaaggtcg
      121 aagcgtccat gaaaaggaac tacattctgg ggctggacat cgggattaca agcgtggggt
      181 atgggattat tgactatgaa acaagggacg tgatcgacgc aggcgtcaga ctgttcaagg
      241 aggccaacgt ggaaaacaat gagggacgga gaagcaagag gggagccagg cgcctgaaac
      301 gacggagaag gcacagaatc cagagggtga agaaactgct gttcgattac aacctgctga
      361 ccgaccattc tgagctgagt ggaattaatc cttatgaagc cagggtgaaa ggcctgagtc
      421 agaagctgtc agaggaagag ttttccgcag ctctgctgca cctggctaag cgccgaggag
      481 tgcataacgt caatgaggtg gaagaggaca ccggcaacga gctgtctaca aaggaacaga
      541 tctcacgcaa tagcaaagct ctggaagaga agtatgtcgc agagctacag ctggaacggc
      601 tgaagaaaga tggcgaggtg agagggtcaa ttaataggtt caagacaagc gactacgtca
      661 aagaagccaa gcagctgctg aaagtgcaga aggcttacca ccagctggat cagagcttca
      721 tcgatactta tatcgacctg ctggagactc ggagaaccta ctatgaggga ccaggagaag
      781 ggagcccctt cggatggaaa gacatcaagg aatggtacga gatgctgatg ggacattgca
      841 cctattttcc agaagagctg agaagcgtca agtacgctta taacgcagat ctgtacaacg
      901 ccctgaatga cctgaacaac ctggtcatca ccagggatga aaacgagaaa ctggaatact
      961 atgagaagtt ccagatcatc ga
//
