LOCUS       sequence-112421-         634 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lysosome-targeted FRET biosensor for monitoring mTORC1 kinase 
            activity; targeted to cytosolic face of lysosomal membrane..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Zhou X, Clister TL, Lowry PR, Seldin MM, Wong GW, Zhang J
  TITLE     Dynamic Visualization of mTORC1 Activity in Living Cells.
  JOURNAL   Cell Rep. 2015 Mar 11. pii: S2211-1247(15)00177-1. doi: 
            10.1016/j.celrep.2015.02.031.
  PUBMED    25772363
REFERENCE   2  (bases 1 to 634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2015 Mar 11. pii: S2211-1247(15)00177-1. doi: 
            10.1016/j.celrep.2015.02.031."
COMMENT     Sequence Label: pcDNA3-Lyso-TORCAR
FEATURES             Location/Qualifiers
     source          1..634
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      73..82
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      77..86
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 aagcttgcgg ccgccaccat ggcggcgccc ggcagcgccc ggcgacccct gctgctgcta
      121 ctgctgttgc tgctgctcgg cctcatgcat tgtgcgtcag cagcaatgtt tatggtgaaa
      181 aatggcaacg ggaccgcgtg cataatggcc aacttctctg ctgccttctc agtgaactac
      241 gacaccaaga gtggccctaa gaacatgacc tttgacctgc catcagatgc cacagtggtg
      301 ctcaaccgca gctcctgtgg aaaagagaac acttctgacc ccagtctcgt gattgctttt
      361 ggaagaggac atacactcac tctcaatttc acgagaaatg caacacgtta cagcgtccag
      421 ctcatgagtt ttgcttataa cttgtcagac acacaccttt tccccaatgc gagctccaaa
      481 gaaatcaaga ctgtggaatc tataactgac atcagggcag atatagataa aaaatacaga
      541 tgtgttagtg gcacccaggt ccacatgaac aacgtgaccg taacgcccca tgatgccacc
      601 atccaggcgt acctttccaa cagcagcttc agca
//
