LOCUS       sequence-112471-         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lim Lab plasmid: AmpR + PLlacO-1::T710 gfp::Asp terminator + ColE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Sagawa S, Shin JE, Hussein R, Lim HN
  TITLE     Paradoxical suppression of small RNA activity at high Hfq 
            concentrations due to random-order binding.
  JOURNAL   Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
  PUBMED    26261213
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Aug 10. pii: gkv777."
COMMENT     Sequence Label: pHL1633
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(14..46)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     RBS             54..76
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        176..280
                     /gene="bla"
                     /label=AmpR promoter
     CDS             465..843
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(499..518)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gcatggatcc gcgacccatt tgctgtccac cagtcatgct agccatatgt atatctcctt
       61 cttaaagtta aacaaaatta tttctagagg taccagtatc ttgttatccg ctcacattta
      121 tggatcagtc gacctgcagt tcgagacgtc aggtggcact tttcggggaa atgtgcgcgg
      181 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
      241 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
      301 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
      361 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
      421 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
      481 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
      541 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
      601 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
      661 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
      721 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
      781 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
      841 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccgg
//
