LOCUS       sequence-112478-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lim Lab plasmid: AmpR + PconShortNoHind::glmZ::Asp terminator + 
            PLlacO-1::T710 hfq::T1 terminator + PLtetO-1::glmS::gfp::T1T2 
            terminator + ColE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Sagawa S, Shin JE, Hussein R, Lim HN
  TITLE     Paradoxical suppression of small RNA activity at high Hfq 
            concentrations due to random-order binding.
  JOURNAL   Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
  PUBMED    26261213
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Aug 10. pii: gkv777."
COMMENT     Sequence Label: pHL2075
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    253..271
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    278..296
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(317..335)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        403..507
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(726..745)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 catgctcgcg ccaacaattc cacacatagt ttttgattcc gatttatatc gttgtcggtc
       61 aacctgtatg cccgtttttt cgggcgcccc gagccttgta gagagtgggg ttatttttat
      121 agttactgct tgtgggaggg agattatgtt atctcctcat cccatgtgac cgggttagcc
      181 ggccagaatc actttttctt taccggacga cgccagcctt ctttctgagt ctgcggcaca
      241 cggtcgacag tatctctatc actgataggg atgtcaatct ctatcactga tagggactcg
      301 aggtgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
      361 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
      421 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
      481 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
      541 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
      601 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
      661 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
      721 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
      781 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcat
//
