LOCUS       sequence-112481-         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + 
            PLtetO-1::RBS (st7) hfq::mCherry::T1 terminator + ColE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Sagawa S, Shin JE, Hussein R, Lim HN
  TITLE     Paradoxical suppression of small RNA activity at high Hfq 
            concentrations due to random-order binding.
  JOURNAL   Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
  PUBMED    26261213
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Aug 10. pii: gkv777."
COMMENT     Sequence Label: pHL391
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    137..153
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        211..315
                     /gene="bla"
                     /label=AmpR promoter
     CDS             500..879
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(534..553)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gcatgctagc tgggaccagg agggacagta ctttaacttt catgttatta accctctgtt
       61 atatgccttt atttgctttt ttatgccact gcatactgat taaccctcat taatcagtcg
      121 gcaagtcgac agtatcttgt tatccgctca caatgtcaat gttatccgct cacatttatg
      181 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
      241 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      301 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
      361 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
      421 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
      481 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
      541 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
      601 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
      661 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
      721 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
      781 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
      841 cgtgacacca cgatgcctgt agcaatggca accaacgttg cgcaa
//
