LOCUS       sequence-112491-         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes mRuby2 as a Type 3a part to be used in the Dueber YTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK039
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      101..135
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(155..865)
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 tactgcgatg agtggcaggg cggggcgtaa tttgatatcg agctcgcttg gactcctgtt
       61 gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc tcggttgccg
      121 ccgggcgttt tttattggtc tggtctcaag aacccttata caattcatcc ataccaccgc
      181 ctaagccagc aaatttggct acggcatgct ctctctgtac aacgaacatt tcgttatcgc
      241 tctcttcgag tctttctagt ctgtggtcta cagcatgtat tcctggcatt ttgatattgc
      301 caacagtctt tttagaacga taagtggtaa caaaactaca agacaaatga cctccaccat
      361 caactttaag cgccatgtgt gtgtagcctc tcaaacctcc atcagctggg tacatcattt
      421 cagtatttgg ttcccaacct ttggtctttt tctgcatcac aggtccgtta gaaggaaaat
      481 tcacccctct tacttgcaca tgatagacaa ggcagccatc ttctaatgaa gtatcttgca
      541 taactgtgac gacaccccca tcttcgtatc ttgtaaccct ttcccatgta aaaccctctg
      601 gaaaagattg cttgaaaaag tctggtatac ctttagggta cttgatgaaa gttcgtgatc
      661 cgtacatgaa tgacgttgcc agaatgtcaa atgcaaatgg tagtggtccg ccttcaatta
      721 ctttaattct catagtttgt gtacccatgt aaggattacc ttcaccttcc cctgtacact
      781 tgaattggtg accattaacg gagccctcca tgacaacttt cattctcatg ttttccttga
      841 ttaactcctc tcctttggac accatatgag accgactacg gttatccaca gaatcagggg
      901 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa gg
//
