LOCUS       sequence-112525-         696 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vector for sgRNAs cloned into the BbsI sites, shRNAs into
            BamHI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Chu VT, Weber T, Wefers B, Wurst W, Sander S, Rajewsky K, Kuhn R
  TITLE     Increasing the efficiency of homology-directed repair for 
            CRISPR-Cas9-induced precise gene editing in mammalian cells.
  JOURNAL   Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198.
  PUBMED    25803306
REFERENCE   2  (bases 1 to 696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198."
COMMENT     Sequence Label: pU6-(BbsI)_CBh-Cas9-T2A-mCherry-H1-(BamHI)
FEATURES             Location/Qualifiers
     source          1..696
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(111..164)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(168..215)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 tcgatctcga actcgtggcc gttcacggag ccctccatgt gcaccttgaa gcgcatgaac
       61 tccttgatga tggccatgtt atcctcctcg cccttgctca ccatgctagc agggccggga
      121 ttctcctcca cgtcaccgca tgttagcaga cttcctctgc cctcaagctt tttctttttt
      181 gcctggccgg cctttttcgt ggccgccggc cttttgtcgc ctcccagctg agacaggtcg
      241 atccgtgtct cgtacaggcc ggtgatgctc tggtggatca gggtggcgtc cagcacctct
      301 ttggtgctgg tgtacctctt ccggtcgatg gtggtgtcaa agtacttgaa ggcggcaggg
      361 gctcccagat tggtcagggt aaacaggtgg atgatattct cggcctgctc tctgatgggc
      421 ttatcccggt gcttgttgta ggcggacagc actttgtcca gattagcgtc ggccaggatc
      481 actctcttgg agaactcgct gatctgctcg atgatctcgt ccaggtagtg cttgtgctgt
      541 tccacaaaca gctgtttctg ctcattatcc tcgggggagc ccttcagctt ctcatagtgg
      601 ctggccaggt acaggaagtt cacatatttg gagggcaggg ccagttcgtt tcccttctgc
      661 agttcgccgg cagaggccag cattctcttc cggccg
//
