LOCUS       sequence-112552-         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses intein-Cas9(C574) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Davis KM, Pattanayak V, Thompson DB, Zuris JA, Liu DR
  TITLE     Small molecule-triggered Cas9 protein with improved genome-editing 
            specificity.
  JOURNAL   Nat Chem Biol. 2015 May;11(5):316-8. doi: 10.1038/nchembio.1793. 
            Epub 2015 Apr 6.
  PUBMED    25848930
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.1793"; journalName: "Nat Chem Biol"; date: 
            "2015-05"; volume: "11"; issue: "5"; pages: "316-8"
COMMENT     Sequence Label: pKMD106h - intein-Cas9(C574)
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..92)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(99..119)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gtgatgatga ccggtcatcc tgcagccttg tcatcgtcat ccttgtaatc gatgtcatga
       61 tctttataat caccgtcatg gtctttgtag tcgctcgaga ctttcctctt cttcttgggg
      121 gatccgtcac ccccaagctg tgacaaatct atccgagttt catataatcc cgtgatggat
      181 tggtgaatca gtgtcgcgtc tagcacctcc ttggtagaag tgtatcgttt gcgatctatc
      241 gttgtgtcaa aatacttgaa tgcggctgga gcgccgaggt tggtaagagt aaacaaatgg
      301 ataatatttt ccgcctgctc acgtatgggt ttatccctgt gcttgttgta tgcgcttaat
      361 actttgtcca gattggcatc agctaggatg actctcttac tgaattccga aatttgctct
      421 atgatttcgt cgagataatg tttgtgctgc tcaacaaaaa gttgcttctg ttcgttatct
      481 tcaggtgaac ctttcaactt ctcgtaatgg gacgctaaat acaggaaatt cacgtattta
      541 gacggtagtg cgagttcgtt ccccttttga agctctccgg cgctagccaa catccgtttt
      601 cggccatttt ctaactcaaa cagactatac tttggtagtt taattatgag atcctttttt
      661 acttccttgt aacctttcgc ctcaaggaag tcgatggggt tcttttcaaa agacgagcgc
      721 tccataatcg ttatccccaa taattctttg actgacttca gtttcttgga ttttcccttc
      781 tcaacttttg ccactactag gacagaatag gcaactgtag ggctatcgaa gccaccgtac
      841 ttt
//
