LOCUS       sequence-112559-         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TRICK translation biosensor with 6x copies of modified PP7 stem 
            loops and 24x MS2 stem loops. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Halstead JM, Lionnet T, Wilbertz JH, Wippich F, Ephrussi A, Singer 
            RH, Chao JA
  TITLE     Translation. An RNA biosensor for imaging the first round of 
            translation from single cells to living animals.
  JOURNAL   Science. 2015 Mar 20;347(6228):1367-671. doi: 
            10.1126/science.aaa3380.
  PUBMED    25792328
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2015-03-20- 20"; volume: "347"; 
            issue: "6228"; pages: "1367-671"
COMMENT     Sequence Label: pmax ponA 6xTRICK 24xMS2SL
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             85..111
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             118..144
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             151..177
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ccggtcgcca ccatgtcgcg gaggcggcac agctacgaga acgatggtgg acaacctcac
       61 aaaaggagga agacgtctgt tgagtaccct tatgatgtcc ctgactacgc tgccgagtat
      121 ccctacgacg tccccgatta tgccgcagaa tacccatacg atgtgccaga ctacgcagct
      181 gagggggggc aagtccagtt gcaagaatct ggacctggtt tggttaaacc atctgagaca
      241 ctttccctaa cctgcacagt cagtggcgca tcaatttcct cctcgcacta ttattggggt
      301 tggattagac aaccacctgg aaagggccca gagtggatag ggtctatgta ctactcgggt
      361 agaacttact ataatcctgc cttgaagtca cgtgttacca tatcctctga taagtcaaaa
      421 aaccaattct ttttaaaact cactagcgtt accgctgctg atacagctgt ctattatgct
      481 gcccgtgagg gcccaacgca ttattatgat aattcaggcc caatacctag cgatgagtat
      541 tttcaacatt ggggccaggg cactttagtt acagtttccg gtggtggcgg ctctggtggc
      601 ggtggcagcg gcggtggtgg ttccggaggc ggcggttctc aggtgcagct gcaggagtcg
      661 ggcccaggac tggtgaagcc ttcggagacc ctgtccctca cctgcactgt ctctggtgcc
      721 tccatcagca gtagtcatta ctactggggc tggattcgcc agcccccagg gaaggggcct
      781 gagtggattg ggagtatgta ttatagtggg agaacgtact acaacccggc c
//
