LOCUS       sequence-112607-        7979 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  phageC31 integrase-expressing helper plasmid for Anopheles 
            transgenesis, vasa promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7979)
  AUTHORS   Volohonsky G, Terenzi O, Soichot J, Naujoks DA, Nolan T, Windbichler
            N, Kapps D, Smidler AL, Vittu A, Costa G, Steinert S, Levashina EA, 
            Blandin SA, Marois E
  TITLE     Tools for Anopheles gambiae Transgenesis.
  JOURNAL   G3 (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 
            10.1534/g3.115.016808.
  PUBMED    25869647
REFERENCE   2  (bases 1 to 7979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 
            10.1534/g3.115.016808."
COMMENT     Sequence Label: pENTR R4-vas2-integrase-R3
FEATURES             Location/Qualifiers
     source          1..7979
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(603..727)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3117..3137
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3156..3182
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3213..5030
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     protein_bind    6042..6165
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(6220..6239)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6221..6239)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6244..6260)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6244..6260)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6362..6381)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             6373..7182
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(6440..6459)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      7329..7917
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7818..7837
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccct acaggtcact aataccatct aagtagttga
      601 ttcatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa
      661 tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcaact tttctataca
      721 aagttggtac cgggcccccc gctagcgtcg acggtatcga tgtagaacgc gagcaaattc
      781 ttttccttcc atgacagcag cagctacagt gggaagccga acgtcagacg tgtttgacat
      841 gccgaactgg gcgggaaaat tacagcgtgc gctttgtttt caagcaaatc acaactcgct
      901 gcaaacaaaa ccgttgagaa attgattgtt ttataatttg tattgtattt tatttgttat
      961 aataaactaa aaagacatac tttttgcata ttttatacat aaaaacatac atgcagcatt
     1021 ataaaacaca tataaaccct ccctgtagag tcccgtatcg aaatcttcca tcctagttgc
     1081 acagtacgac ggacgagtag gccgtgtccg tgcaaattcc agcttttagc agtcttttgc
     1141 tcggagcact cgcggcgagt cggaggtttc tgctgaggtg cttagcgcta aattagccaa
     1201 ttgcttttgc aagtgaaata accagccgaa tagtacttca aaactcaggt aagtgaacta
     1261 gttttataga acaaatgttt gtttgttaga agttagtgaa gtgtttgtga aaaaaatctc
     1321 tcatttcggc aaaactaacg taactgattt caaattgaat tattgttttg tgatgttata
     1381 ttatttcatc cagttgatta gtattttctt agttatgttc aaaatacagt taaattaaat
     1441 ttcatttcat ttactcataa aataatctct tggcttattt aatttttctc gaattcgctt
     1501 gtattgttca gtagcacgcg ccattcgccc tttgtttcat tttgtacctg ctcccactaa
     1561 cacactggca gtgcgaaaca aaagccttcg cacgcgttgc tggtattaga gtgtgtgcgt
     1621 gtgtgtgttg agcgctctgt caaaatcggc tgttgccgcc ggtaccgaaa ttgcctgttc
     1681 gcacgctgtt cgtaaacatt ccgtggtgtg tatcgtgtgt tgtgcatgtt gcgcgcctcc
     1741 ccccttttga tagcaggctg ccgtggctgc cgtggtgtgt ggcgcagttg agtttttgga
     1801 ttaattttct aaggaaatgg cacgagaaga gcggtggcag tgtgttggtt tgctctgtcc
     1861 cttcctttct gtgtgaagtg ttcttacagc acagcacgta tccaccaccg cacacagagc
     1921 aggcaaggaa gtggaagtga acaagtgtgc tgcgcatgca tgtgtgtggg gggcatttta
     1981 gctgagatcg tcgttatttg agaagcggta taggggccag tcggtgtcga cgtacggaag
     2041 cggtttagtt ttaatccaag cgtatcccgt cgtggagtgg ttgtgtggct ctgtgtgctc
     2101 tcatatcagt tccagagtga ggttagtaga atcacagtcc ttggcctttt tcgttacaag
     2161 atatccagaa ggatggcgtt atttccacag cttaccatgg tgctcttgtt tgctcgaatc
     2221 aggggagaaa aacagtttcg tgtttcatga accgcagttg gcactggagc ggattcaaaa
     2281 gtcttcgata tgcaatagat aagagagtcg ttggggcata gttgggaagc ctttccgaga
     2341 tgtggagttt ccgagaggag aaatggtgct ttcgtgcacg ttccgggaca gcgggccccg
     2401 cgaagagcat ctcgttgtcg ttcatccggc aataattgat gcgaaaagcg cgcgcgccac
     2461 tggcttagcg cagtgtacac agtgatattc acctacacac acagaggcac acgccttcac
     2521 acgcgcgcgt gcttcaaagg ctacttcggt ggcggtgtgt gaggtcgctt gcaatggaca
     2581 atgaaaattt cgctggaaaa taccatcgtc tctttaggtt gcaatgggtg cgggtagagc
     2641 ggtggtcgtc gatattggtg gtgtagtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg
     2701 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg
     2761 tgcaacggca attatttttt gtaatatttc gaccatcttt ctttctctct ctccacgtgc
     2821 tgctgctgtt gctgctgctg ctgcattgca tgttccacta ttcctctcgg tttgtgcctg
     2881 cggacgccat tgctagtcga aagagagtcg ccgttagtcg cgcttcgagc aacggacacg
     2941 ttttttggtt gaaaccaaca gcttttttca tcttcgggag acacacagat ctcgaatcgt
     3001 acattcccat aaggagaatt gtcatcttcc ggtgaataaa gaaaggaaac ggatccggaa
     3061 acatgggatc atcatcagac gacgaagcta cggccgatgc gcagcatgca gcaccaccaa
     3121 aaaaaaaacg aaaagtagaa gacccacgat ttatgtaccc atacgatgtt cctgactatg
     3181 cgggtatgaa aaacatcaaa aaaaaccagg ttatggacac gtacgcgggt gcttacgacc
     3241 gtcagtcgcg cgagcgcgaa aattcgagcg cagcaagccc agcgacacag cgtagcgcca
     3301 acgaagacaa ggcggccgac cttcagcgcg aagtcgagcg cgacgggggc cggttcaggt
     3361 tcgtcgggca tttcagcgaa gcgccgggca cgtcggcgtt cgggacggcg gagcgcccgg
     3421 agttcgaacg catcctgaac gaatgccgcg ccgggcggct caacatgatc attgtctatg
     3481 acgtgtcgcg cttctcgcgc ctgaaggtca tggacgcgat tccgattgtc tcggaattgc
     3541 tcgccctggg cgtgacgatt gtttccactc aggaaggcgt cttccggcag ggaaacgtca
     3601 tggacctgat tcacctgatt atgcggctcg acgcgtcgca caaagaatct tcgctgaagt
     3661 cggcgaagat tctcgacacg aagaaccttc agcgcgaatt gggcgggtac gtcggcggga
     3721 aggcgcctta cggcttcgag cttgtttcgg agacgaagga gatcacgcgc aacggccgaa
     3781 tggtcaatgt cgtcatcaac aagcttgcgc actcgaccac tccccttacc ggacccttcg
     3841 agttcgagcc cgacgtaatc cggtggtggt ggcgtgagat caagacgcac aaacaccttc
     3901 ccttcaagcc gggcagtcaa gccgccattc acccgggcag catcacgggg ctttgtaagc
     3961 gcatggacgc tgacgccgtg ccgacccggg gcgagacgat tgggaagaag accgcttcaa
     4021 gcgcctggga cccggcaacc gttatgcgaa tccttcggga cccgcgtatt gcgggcttcg
     4081 ccgctgaggt gatctacaag aagaagccgg acggcacgcc gaccacgaag attgagggtt
     4141 accgcattca gcgcgacccg atcacgctcc ggccggtcga gcttgattgc ggaccgatca
     4201 tcgagcccgc tgagtggtat gagcttcagg cgtggttgga cggcaggggg cgcggcaagg
     4261 ggctttcccg ggggcaagcc attctgtccg ccatggacaa gctgtactgc gagtgtggcg
     4321 ccgtcatgac ttcgaagcgc ggggaagaat cgatcaagga ctcttaccgc tgccgtcgcc
     4381 ggaaggtggt cgacccgtcc gcacctgggc agcacgaagg cacgtgcaac gtcagcatgg
     4441 cggcactcga caagttcgtt gcggaacgca tcttcaacaa gatcaggcac gccgaaggcg
     4501 acgaagagac gttggcgctt ctgtgggaag ccgcccgacg cttcggcaag ctcactgagg
     4561 cgcctgagaa gagcggcgaa cgggcgaacc ttgttgcgga gcgcgccgac gccctgaacg
     4621 cccttgaaga gctgtacgaa gaccgcgcgg caggcgcgta cgacggaccc gttggcagga
     4681 agcacttccg gaagcaacag gcagcgctga cgctccggca gcaaggggcg gaagagcggc
     4741 ttgccgaact tgaagccgcc gaagccccga agcttcccct tgaccaatgg ttccccgaag
     4801 acgccgacgc tgacccgacc ggccctaagt cgtggtgggg gcgcgcgtca gtagacgaca
     4861 agcgcgtgtt cgtcgggctc ttcgtagaca agatcgttgt cacgaagtcg actacgggca
     4921 gggggcaggg aacgcccatc gagaagcgcg cttcgatcac gtgggcgaag ccgccgaccg
     4981 acgacgacga agacgacgcc caggacggca cggaagacgt agcggcgtag tgtacccccc
     5041 tcgagcttgg ggtggggttg ttatgtgttg cgaacgagag tggatctctc tcgacatttc
     5101 cttatttttt ttttctattg ttaacactta caacgaaact tcggaagaga agtttcctca
     5161 ttcgaaacga ggagtcaaac tcctttcctt gcttttgtga catgcatgat tattctttca
     5221 tttactgacg taacgatgta aaacacacag aagaagcatg acacacagca aagaattgtt
     5281 cgttaataaa acgttgatga aaactttgaa aacataagaa cttgcacttt tattctataa
     5341 ttcgtgaaag cttgcaccga ttgtctttca ttattcaatg taatgtactg aaaggtgatt
     5401 tttcgcactt gtatactcta gaactgaagt attctaacaa tacgtcacct ttaggtccat
     5461 tccaggataa aatacacaag tgaaggtagt tgtacaaagt acttagacta acccaggttt
     5521 caaaaaagat aacgacgaat tgagcgtaac tgcacaaagc ccgttcattg tcaacatatc
     5581 gttcaggggt cttcaaactt tattatcagc ccgcgggccg cttagagcac catcttgatg
     5641 tatcctttat ctagtggctt atcgtttatg tctcgtttgg ctaagaaaat actaataaca
     5701 tattcaattt acggagcggt accatggtac aaacgtcata ctgctagact tgacaacatg
     5761 cccatcgagg gttcttacct caaatggacc gatcccttgt aactggcggc gtaaactcag
     5821 taaattccaa aagccagtat aggccaccat gaccgtctaa ctattccaaa aaggagaata
     5881 tttgaaattt ctatatctat agtattatta ctatatctat atgtttcttt agtacaatat
     5941 tagagcttga ttattttcct gaaaattgta aaatatgcat gttagatggt tgatacatcg
     6001 ttttaaaaaa aactgctgtt aatggccaca ttttctcgag acaactttgt ataataaagt
     6061 tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa
     6121 acagactaca taatactgta aaacacaaca tatccagtca ctatgaatca actacttaga
     6181 tggtattagt gacctgtact gcagggcggc cgcgatatcc cctatagtga gtcgtattac
     6241 atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt
     6301 gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat
     6361 acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt aaattccaac
     6421 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
     6481 acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
     6541 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
     6601 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
     6661 actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
     6721 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
     6781 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
     6841 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
     6901 tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat
     6961 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
     7021 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
     7081 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
     7141 aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg
     7201 ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcaagctc atgaccaaaa
     7261 tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa
     7321 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     7381 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     7441 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     7501 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     7561 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     7621 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     7681 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     7741 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     7801 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     7861 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     7921 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgtt
//
