LOCUS       sequence-112629-         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV packaging vector for Cre-dependent, hSyn driven KOR DREADD 
            expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Vardy E, Robinson JE, Li C, Olsen RH, DiBerto JF, Giguere PM, 
            Sassano FM, Huang XP, Zhu H, Urban DJ, White KL, Rittiner JE, 
            Crowley NA, Pleil KE, Mazzone CM, Mosier PD, Song J, Kash TL, 
            Malanga CJ, Krashes MJ, Roth BL
  TITLE     A New DREADD Facilitates the Multiplexed Chemogenetic Interrogation 
            of Behavior.
  JOURNAL   Neuron. 2015 Apr 29. pii: S0896-6273(15)00289-5. doi: 
            10.1016/j.neuron.2015.03.065.
  PUBMED    25937170
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2015 Apr 29. pii: S0896-6273(15)00289-5. doi: 
            10.1016/j.neuron.2015.03.065."
COMMENT     Sequence Label: pAAV-hSyn-dF-HA-KORD-IRES-mCitrine
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      136..145
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(142..144,145..147,148..660)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(187..208)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(448..467)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt
       61 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
      121 aaaacacgat gataagccac catggtgagc aagggcgagg agctgttcac cggggtggtg
      181 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
      241 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
      301 ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgat gtgcttcgcc
      361 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
      421 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
      481 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
      541 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
      601 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
      661 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
      721 gtgctgctgc ccgacaacca ctacctgagc taccagtcca aactgagcaa agaccccaac
      781 gagaagcgcg atcacatggt cctgctggag ttc
//
