LOCUS       sequence-112698-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Spacer as a Type 234 part to be used in the Dueber YTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK048
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      98..132
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     primer_bind     complement(381..400)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgcgatgagt ggcagggcgg ggcgtaattt gatatcgagc tcgcttggac tcctgttgat
       61 agatccagta atgacctcag aactccatct ggatttgttc agaacgctcg gttgccgccg
      121 ggcgtttttt attggtctgg tctcacagct cgatctcgag ttaggatcct cacatagatc
      181 tctagtcgtt tgagaccgac tacggttatc cacagaatca ggggataacg caggaaagaa
      241 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
      301 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      361 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      421 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      481 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      541 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      601 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      661 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      721 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      781 cttcggaaaa agagttggta gctcttgatc cggcaaacaa
//
