LOCUS       sequence-112742-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes ConR3 as a Type 5 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK069
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      91..125
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     primer_bind     complement(506..525)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtggcaggg cggggcgtaa tttgatatcg agctcgcttg gactcctgtt gatagatcca
       61 gtaatgacct cagaactcca tctggatttg ttcagaacgc tcggttgccg ccgggcgttt
      121 tttattggtc tggtctcatg tactgcagtg cactagtgta ctgggttcga gttccctgat
      181 ccgtatgtcg aatggctacc aggctgacga cgatgatatg agcgtgcatc ggcatcttgc
      241 ggtgttgatt agctgccact ggtggctgtc ctacatcagc ctcatgtggt ctaggtctgc
      301 cgtctcagaa ccagctgaga ccgactacgg ttatccacag aatcagggga taacgcagga
      361 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
      421 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
      481 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
      541 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
      601 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
      661 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
      721 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
      781 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
      841 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
//
