LOCUS       sequence-112746-         207 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of His-tagged Med25-VBD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 207)
  AUTHORS   Sela D, Chen L, Martin-Brown S, Washburn MP, Florens L, Conaway JW, 
            Conaway RC
  TITLE     Endoplasmic reticulum stress-responsive transcription factor 
            ATF6alpha directs recruitment of the Mediator of RNA polymerase II 
            transcription and multiple histone acetyltransferase complexes.
  JOURNAL   J Biol Chem. 2012 Jun 29;287(27):23035-45. doi: 
            10.1074/jbc.M112.369504. Epub 2012 May 10.
  PUBMED    22577136
REFERENCE   2  (bases 1 to 207)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.369504"; journalName: "J Biol Chem"; date: 
            "2012-06-29- 29"; volume: "287"; issue: "27"; pages: "23035-45"
COMMENT     Sequence Label: pET21a-Med25-VBD-6His
FEATURES             Location/Qualifiers
     source          1..207
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             23..55
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 actttaagaa ggagatatac atatggctag catgactggt ggacagcaaa tgggtcgcgg
       61 atcctcagtc tccaataagc ttctggcctg gagcggggtc ctggagtggc aagagaaacc
      121 caaacctgcc tcagtggatg ccaacaccaa gctgacgcgg tcactgccct gccaggtcta
      181 cgtgaatcat ggcgagaacc tgaagac
//
