LOCUS       sequence-112752-         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes KanamycinR as a Type 6 part to be used in the Dueber YTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK077
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      92..126
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     terminator      complement(144..341)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 gagtggcagg gcggggcgta atttgatatc gagctcgctt ggactcctgt tgatagatcc
       61 agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc gccgggcgtt
      121 ttttattggt ctggtctcaa ctccagtata gcgaccagca ttcacatacg attgacgcat
      181 gatattactt tctgcgcact taacttcgca tctgggcaga tgatgtcgag gcgaaaaaaa
      241 atataaatca cgctaacatt tgattaaaat agaacaacta caatataaaa aaactataca
      301 aatgacaagt tcttgaaaac aagaatcttt ttattgtcag ttactttaga aaaactcatc
      361 gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa
      421 aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc
      481 ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc
      541 gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa
      601 tggcaaaagc ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc
      661 atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagccagacg
      721 aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag
      781 gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg
      841 gaatgctgt
//
