LOCUS       sequence-112761-         757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes AmpR-ColE1 as a Type 8 part to be used in the Dueber YTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK083
FEATURES             Location/Qualifiers
     source          1..757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(175..202)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(218..289)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
       61 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
      121 taggtgaaga tcctttttga taatcgcggc cgctcggtga gaccgactta taaacgcaga
      181 aaggcccacc cgaaggtgag ccagtgtgac tctagtagag agcgttcacc gacaaacaac
      241 agataaaacg aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctggaga
      301 tccttattaa gcaccggtgg agtgacgacc ttcagcacgt tcgtactgtt caacgatggt
      361 gtagtcttcg ttgtgggagg tgatgtccag tttgatgtcg gttttgtaag cacccggcag
      421 ctgaaccggt tttttagcca tgtaggtggt tttaacttca gcgtcgtagt gaccaccgtc
      481 tttcagtttc agacgcattt tgatttcacc tttcagagca ccgtcttccg ggtacatacg
      541 ttcggtggaa gcttcccaac ccatggtttt tttctgcata accggaccgt cggacgggaa
      601 gttagtacca cgcagtttaa ctttgtagat gaactcaccg tcttgcaggg aggagtcctg
      661 ggtaacggta acaacaccac cgtcttcgaa gttcataaca cgttcccatt tgaaaccttc
      721 cgggaaggac agtttcaggt agtccgggat gtcagcc
//
