LOCUS       sequence-112801-         936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a polyspecific aminoacyl-tRNA synthetase and four copies of 
            amber suppression tRNAs for unnatural amino acid incorporation in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Chen ZJ, Ren W, Wright QE, Ai HW
  TITLE     Genetically encoded fluorescent probe for the selective detection of
            peroxynitrite.
  JOURNAL   J Am Chem Soc. 2013 Oct 9;135(40):14940-3. doi: 10.1021/ja408011q. 
            Epub 2013 Sep 26.
  PUBMED    24059533
REFERENCE   2  (bases 1 to 936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja408011q";
            journalName: "J Am Chem Soc"; date: "2013-10-9- 9"; volume: "135"; 
            issue: "40"; pages: "14940-3"
COMMENT     Sequence Label: pMAH-POLY
FEATURES             Location/Qualifiers
     source          1..936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(173..277)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        complement(401..491)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(461..480)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        complement(603..851)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(661..680)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(831..851)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
       61 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
      121 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
      181 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
      241 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga ctcgactcgc
      301 tagtgttcca aaaaatggtg ggggacggat tcgaaccgcc gaacccaaag ggagcggatt
      361 tagagtccgc ggcgtttggc cacttcgcta ccccaccaga gtggtctcat acagaactta
      421 taagattccc aaatccaaag acatttcacg tttatggtga tttcccagaa cacatagcga
      481 catgcaaata tgaattgatg actgcctgat ggagaaaaaa tggtggggga cggattcgaa
      541 ccgccgaacc caaagggagc ggatttagag tccgcggcgt ttggccactt cgctacccca
      601 ccggtgtttc gtcctttcca caagatatat aaagccaaga aatcgaaata ctttcaagtt
      661 acggtaagca tatgatagtc cattttaaaa cataatttta aaactgcaaa ctacccaaga
      721 aattattact ttctacgtca cgtattttgt actaatatct ttgtgtttac agtcaaatta
      781 attctaatta tctctctaac agccttgtat cgtatatgca aatatgaagg aatcatggga
      841 aataggccct cttcctgccc gaccttcgcg atgtacgggc cagatatacg ccaatattgg
      901 ccattagcca tattattcat tggttatata gcataa
//
