LOCUS       sequence-112807-         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TpR mini-Tn7 vector, mobilizable, for RFP tagging bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Choi KH, Schweizer HP
  TITLE     mini-Tn7 insertion in bacteria with single attTn7 sites: example 
            Pseudomonas aeruginosa.
  JOURNAL   Nat Protoc. 2006;1(1):153-61.
  PUBMED    17406227
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(1)"; pages: "153-61"
COMMENT     Sequence Label: pUC18T-mini-Tn7T-Tp-dsRedExpress
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      82..176
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      279..365
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    complement(497..544)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        join(596..601,602..618,619..624)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 gagggccgcc accacctgtt cctgtagaag ccgaattcca gcacactggc ggccggtacc
       61 aagcttaatt agctgagctt ggactcctgt tgatagatcc agtaatgacc tcagaactcc
      121 atctggattt gttcagaacg ctcggttgcc gccgggcgtt ttttattggt gagaatccaa
      181 gctagactgc gatgagtggc agggcggggc gtaatttttt taaggcagtt attggtgccc
      241 ttaaacgcct ggggtaatga ctctctagct tgaggcatca aataaaacga aaggctcagt
      301 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga
      361 caaatccgcc gctaggagct tgcggcccgc cttcgcgagg taccgggccc aagcttctcg
      421 aggaattcct gcagcccggg ggatccacta gtgagctcat gcatgatcga gctcgaatta
      481 gcttcaaaag cgctctgaag ttcctatact ttctagagaa taggaacttc ggaataggaa
      541 cttcaagatc ccctgattcc ctttgtcaac agcaatggat aattcacgaa cccagttgac
      601 ataagcctgt tcggttcgta aactgtaatg caagtagcgt atgcgctcac gcaactggtc
      661 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
      721 tgactg
//
