LOCUS       sequence-112830-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a Fluorescent Reporter for Hydrogen Sulfide in Mammalian 
            Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Chen ZJ, Ai HW
  TITLE     A highly responsive and selective fluorescent probe for imaging 
            physiological hydrogen sulfide.
  JOURNAL   Biochemistry. 2014 Sep 23;53(37):5966-74. doi: 10.1021/bi500830d. 
            Epub 2014 Sep 5.
  PUBMED    25141269
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/bi500830d";
            journalName: "Biochemistry"; date: "2014-09-23- 23"; volume: "53"; 
            issue: "37"; pages: "5966-74"
COMMENT     Sequence Label: pcDNA3-hsGFP
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    16..604
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             122..133
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     531..551
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(620..637)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atagggccct ctagagcggg gaggcggccc aaagggagat ccgactcgtc tgagggcgaa
       61 ggcgaagacg cggaagaggc cgcagagccg gcagcaggcc gcgggaagga aggtccgctg
      121 gattgagggc cgaagggacg tagcagaagg acgtcccgcg cagaatccag gtggcaacac
      181 aggcgagcag ccaaggaaag gacgatgatt tccccgacaa caccacggaa ttgtcagtgc
      241 ccaacagccg agcccctgtc cagcagcggg caaggcaggc ggcgatgagt tccgccgtgg
      301 caatagggag ggggaaagcg aaagtcccgg aaaggagctg acaggtggtg gcaatgcccc
      361 aaccagtggg ggttgcgtca gcaaacacag tgcacaccac gccacgttgc ctgacaacgg
      421 gccacaactc ctcataaaga gacagcaacc aggatttata caaggaggag aaaatgaaag
      481 ccatacggga agcaatagca tgatacaaag gcattaaagc agcgtatcca catagcgtaa
      541 aaggagcaac atagttaaga ataccagtca atctttcaca aattttgtaa tccagaggtt
      601 gattgtcgac ctcgagttaa tggtgatggt gatggtgcca gttgtactcc agcttgtgcc
      661 ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg
      721 tgtcgccctc gaacttcacc tcggcgcggg tcttgtaggt gccgtcgtcc ttgaagaaga
      781 tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca
      841 tgtggtcggg gtagcgggag aagcactgca cgccctaggt caaggtggtc acgagggtgg
//
