LOCUS       sequence-112834-        1090 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral expression of CCL20 shRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Affo S, Morales-Ibanez O, Rodrigo-Torres D, Altamirano J, Blaya D, 
            Dapito DH, Millan C, Coll M, Caviglia JM, Arroyo V, Caballeria J, 
            Schwabe RF, Gines P, Bataller R, Sancho-Bru P
  TITLE     CCL20 mediates lipopolysaccharide induced liver injury and is a 
            potential driver of inflammation and fibrosis in alcoholic 
            hepatitis.
  JOURNAL   Gut. 2014 Nov;63(11):1782-92. doi: 10.1136/gutjnl-2013-306098. Epub 
            2014 Jan 10.
  PUBMED    24415562
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1136/gutjnl-2013-306098"; journalName: "Gut"; date: "2014-11"; 
            volume: "63"; issue: "11"; pages: "1782-92"
COMMENT     Sequence Label: shCCL20
FEATURES             Location/Qualifiers
     source          1..1090
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..19
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        300..548
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     300..320
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     471..490
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     ncRNA           603..724
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           750..764
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           790..916
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        complement(994..1012)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccctttatcc agccctcact ccttctctag gcgccggaat tagatctctc gataataggg
       61 gacggatccc cccgagtcca acacccgtgg gaatcccatg ggcaccatgg cccctcgctc
      121 caaaaatgct ttcgcgtcgc gcagacactg ctcggtagtt tcggggatca gcgtttgagt
      181 aagagcccgc gtctgaaccc tccgcgccgc cccggcccca gtggaaagac gcgcaggcaa
      241 aacgcaccac gtgacggagc gtgaccgcgc gccgagcgcg cgccagggtc gggcaggaag
      301 agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga
      361 taattagaat taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa
      421 agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata
      481 tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
      541 gaaacaccgt gctcgcttcg gcagcacata tactagtcga ctagggataa cagggtaatt
      601 gtttgaatga ggcttcagta ctttacagaa tcgttgcctg cacatcttgg aaacacttgc
      661 tgggattact tcttcaggtt aacccaacag aaggctcgag aaggtatatt gctgttgaca
      721 gtgagcgcaa gcagaactgg gtgaaaatag tgaagccaca gatgtatttt cacccagttc
      781 tgcttttgcc tactgcctcg gaattcaagg ggctacttta ggagcaatta tcttgtttac
      841 taaaactgaa taccttgcta tctctttgat acatttttac aaagctgaat taaaatggta
      901 taaattaaat cacttttttc aattggaaga ctgtggttct gtatgagtcc attttgtcgt
      961 ttaatctctg gtgcaagccg tgtggattaa catcctatag tgagtcgtat taaattacgc
     1021 gttcgaccga ataaatacct gtgacggaag atcacttcgc agaataaata aatcctggtg
     1081 tccctgttga
//
