LOCUS       sequence-112859-        1956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses K29-/K33-specific TRABID NZF1 (aa 1-33) in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Michel MA, Elliott PR, Swatek KN, Simicek M, Pruneda JN, Wagstaff 
            JL, Freund SM, Komander D
  TITLE     Assembly and specific recognition of k29- and k33-linked 
            polyubiquitin.
  JOURNAL   Mol Cell. 2015 Apr 2;58(1):95-109. doi: 
            10.1016/j.molcel.2015.01.042. Epub 2015 Mar 5.
  PUBMED    25752577
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.01.042"; journalName: "Mol Cell"; date: 
            "2015-04-2- 2"; volume: "58"; issue: "1"; pages: "95-109"
COMMENT     Sequence Label: pOPINK-TRABID (NZF1, aa 1-33)
FEATURES             Location/Qualifiers
     source          1..1956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             174..191
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(244..262)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      258..305
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      342..797
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(429..448)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     639..660
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     gap             1409..1425
                     /estimated_length=17
     gap             1450..1466
                     /estimated_length=17
     gap             1471..1485
                     /estimated_length=15
     gap             1496..1512
                     /estimated_length=17
     gap             1602..1615
                     /estimated_length=14
     gap             1654..1668
                     /estimated_length=15
     gap             1672..1699
                     /estimated_length=28
     gap             1703..1715
                     /estimated_length=13
     gap             1717..1731
                     /estimated_length=15
     gap             1734..1843
                     /estimated_length=110
     gap             1846..1855
                     /estimated_length=10
     gap             1869..1880
                     /estimated_length=12
     gap             1893..1902
                     /estimated_length=10
     gap             1930..1943
                     /estimated_length=14
ORIGIN
        1 gggnnantca atcggatctg acagcggtct ggaagttctg ttnagggccc gatgtcagaa
       61 cgtggaatta agtgggcttg tgaatattgt acgtatgaaa actggccatc tgcaatcaag
      121 tgtactatgt gtcgtgccca aagacctagt taaagctttc tagaccattt aaacaccacc
      181 accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg
      241 ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt
      301 ttttgctgaa aggaggaact atatccggat tggcgaatgg gacgcgccct gtagcggcgc
      361 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      421 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      481 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      541 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      601 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
      661 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
      721 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
      781 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
      841 ttatttttct aaatacattc aaatatgtat ccgctcatga attaattctt agaaaaactc
      901 atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg
      961 aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag
     1021 atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc
     1081 ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtgn
     1141 naatggcaaa agtttatgca ttnctttcca gacttgttca acaggccagc cattacgctc
     1201 gtcatcaaaa tcactcggat caaccaaacc gttattcatt cgngnnnggc cctgaacnnn
     1261 annaantacc ngntccntgt taaanggaaa tnnnaannnn gnnncgaatg gaaccggnna
     1321 nnnaacttgn nagcnnnann nanannttnn cnnnannnnn nannnnnnnn aanntggnaa
     1381 nnnnnnttcc cggnnannnn ngngnggann nnnnnnnnnn nnnnnannnn anaannnnnn
     1441 nnngnngaan nnnnnnnnnn nnnnnngttt nnnnnnnnnn nnnnngaant ttgngnnnnn
     1501 nnnnnnnnnn nnaaaaannn nggnncnnnn gcnnnacaaa aaanngnnnn ngnnngnnna
     1561 tagnnncnnn tnntnnaaaa ncngnnnnta nnnnnnnnna gnnnnnnnnn nnnnnannnn
     1621 nnnngtnnnn ngnnntnaan nnanatnaan tnannnnnnn nnnnnnnnta annnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnna nannnnnnnn nnnnntnnnn nnnnnnnnnn naannnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaannnnn nnnnnaannn
     1861 naaaaaaann nnnnnnnnnn annnannnnn aannnnnnnn nnanaannnn nnnaannaan
     1921 aaaaaaaaan nnnnnnnnnn nnnannnnna annnaa
//
