LOCUS       sequence-112890-         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  source of TpR FRT cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer 
            RR, Schweizer HP
  TITLE     A Tn7-based broad-range bacterial cloning and expression system.
  JOURNAL   Nat Methods. 2005 Jun;2(6):443-8.
  PUBMED    15908923
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2005-06"; volume: "2(6)"; pages: "443-8"
COMMENT     Sequence Label: pFTP1
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    25..72
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(134..370)
                     /codon_start=1
                     /gene="dhfrIIb from plasmid R388"
                     /product="E. coli plasmid-associated dihydrofolate 
                     reductase"
                     /label=TpR
                     /note="confers resistance to trimethoprim"
                     /translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVGW
                     YCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
     promoter        complement(join(696..701,702..718,719..724))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 taccgagctc gaattgggga tcttgaagta cctattccga agttcctatt ctctagaaag
       61 tataggaact tcagagcgct tttgaagctg atgtgcttaa aaacttactc aatggaataa
      121 ttctagataa ttcttaggcc acacgttcaa gtgcagccac aggataaatt tgcactgagc
      181 ctgggtggga ttcggactcg accgcatagc cttcaggagt gagttttgtg caataccaac
      241 cgacgacttg accctgccaa gcggcaccag atttcttgcg tacgcgatcc cctaagccaa
      301 aggtggcact caggggaagc gcaaactgcc ctgcaacggg agcgttggct tcatcgctac
      361 tttgacccat gtcgaatcct tcttgtgaat ctattatggc gacaagcaaa tcgagctctg
      421 actgcctacc ccacaacaac tatcagaaag caccagcaca acggctgcct aactttgttt
      481 tagggcgact gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca
      541 cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacaaaa aaacagtcat
      601 aacaagccat gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc aaggttctgg
      661 accagttgcg tgagcgcata cgctacttgc attacagttt acgaaccgaa caggcttatg
      721 tcaact
//
