LOCUS       sequence-113013-         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of RFP in mammalian cells. Control vector for 
            FUW-Ephrin-RFP vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Cortina C, Palomo-Ponce S, Iglesias M, Fernandez-Masip JL, Vivancos 
            A, Whissell G, Huma M, Peiro N, Gallego L, Jonkheer S, Davy A, 
            Lloreta J, Sancho E, Batlle E
  TITLE     EphB-ephrin-B interactions suppress colorectal cancer progression by
            compartmentalizing tumor cells.
  JOURNAL   Nat Genet. 2007 Nov;39(11):1376-83. Epub 2007 Sep 30.
  PUBMED    17906625
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Genet."; date: "2007-11"; volume: "39(11)"; pages: "1376-83. Epub 
            2007 Sep 30"
COMMENT     Sequence Label: FUW-ubiquitin-SV40-RFP
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(164..374)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      170..305
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(175..472)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        322..513
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     470..490
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     misc_feature    612..727
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
ORIGIN
        1 gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc ggccctcgcc ctcgccctcg
       61 atctcgaact cgtggccgtt cacggagccc tccatgcgca ccttgaagcg catgaactcc
      121 ttgatgacgt cctcggagga ggccatggtg ggctagctgc aaaagcctag gcctccaaaa
      181 aagcctcctc actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa
      241 ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc
      301 gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc
      361 atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga
      421 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga
      481 cacacattcc acagctggtt ctttccgcct cagaaggtac ctaaccaagt tcctctttca
      541 gaggttattt caggccatgg tgctgcgcga ttaaccaaac tggatctctg ctgtccctgt
      601 aataaacccg aaaattttga atttttgtaa tttgtttttg taattcttta gtttgtatgt
      661 ctgttgctat tatgtctact attctttccc ctgcactgta ccccccaatc cccccttttc
      721 ttttaaa
//
