LOCUS       sequence-11367-e        9160 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9160)
  AUTHORS   Hesselson D, Anderson RM, Beinat M, Stainier DY
  TITLE     Distinct populations of quiescent and proliferative pancreatic 
            beta-cells identified by HOTcre mediated labeling.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Sep 1. 106(35):14896-901.
  PUBMED    19706417
REFERENCE   2  (bases 1 to 9160)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Sep 1. 106(35):14896-901."
COMMENT     Sequence Label: hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-ce...
FEATURES             Location/Qualifiers
     source          1..9160
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     26..45
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        26..44
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(1635..1668)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             1692..2402
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1837..1855)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2105..2124
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     intron          2486..2551
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2681..2701
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3126..3260
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(3315..3348)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(3785..3787,3788..3790,3791..4504)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3785..3787,3788..3790,3791..4501)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    4627..4748
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    4791..4998
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     4987..5004
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(join(5022..5735,5736..5738,5739..5741))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6325..6345)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6325..6343)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6376..6393)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6376..6392)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6385..6407)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(6533..6987)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6669..6690)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6881..6900
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7014..7118
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7119..7187,7188..7979)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7337..7356)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8150..8738
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8639..8658
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8892..8909
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9026..9047
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9062..9067,9068..9085,9086..9092)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9100..9116
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9105..9127
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9124..9140
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9124..9140
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tagggataac agggtaatgc gcgcgtaata cgactcacta tagggcgaat tggagctcat
       61 tctcaggggt gtcgcttggt tatttccaaa aatcaaatta attttattaa actattagaa
      121 cgagcatgtt ttgtctatat gctacagaag ataaaaaata ataggagtta acagttataa
      181 aacaacacat ttgtttctat tgattgttga ccacactggg gtctcattaa gttagattaa
      241 agacacacta actgggtcaa aagcagcaga ttgatttcat agcaccaggg taaactttct
      301 aacactttta cggcaatcat atacattaaa attaaataca gaccacgact gaacaaggag
      361 gatgatctcc aatattaaac aaagagactt gtgcctattt ctctgagggt aaacatgacc
      421 tctcaagtta gcaagttgtt tttaacacta caaaaatagt taagacctgc aatcccagaa
      481 taaagtattg gttttaacca atcaatatag tacagtaaac atccatttgt tttgttgaaa
      541 cgttaaacaa atctgaccaa agctattagc ttatataaaa caggtttgcc ttctatgtag
      601 ctgaaaacac cacaggcccg attttgctac tgtgtaaaac atttcagcaa gattttttta
      661 ttgcattttt ttttactgaa tcgttcaaac attttatcat tttagtttgt tcattcattg
      721 caactggaaa aacaacacat cacacaaccg cacatatttc agcaataagt acaataaaac
      781 actcaaataa aaaaaacatt ttaaatctct ttgtattttt gaccgctgtt tcgcgtaatt
      841 tcacggtaaa actctggaaa tctccactac attcctctca gcggctcctc tcaatgacag
      901 ctgaagaagt gacggcggct gcctgctgtg ttttgattgg tcgaattcac tggaggcttc
      961 cagaacagtg tagagtctga acgggtgcgc gctctgctgt atttaaaggg cgaaagagag
     1021 accgcagaga aactcaaccg aagagaagcg acttgacaaa gaagaaaaga gcagcctgac
     1081 aggacttttc cccgacgagg tgtttattcg ctctatttaa gaatctactg taaggtaagt
     1141 ctcaatatat tgtactctat tggctaatca gaattatata gagattatat gtacttaatg
     1201 tcaaaaaatc aactttgtat atgtaatctt tttacatgtg gactgcctat gttcatctta
     1261 ttttaggtct actagaaaat tatatttccc gttttcacaa taaggatttt aaaaaaagca
     1321 atgaacagac gggcatttac tttatgttgc tgacattatt ttatatgagc ataataacca
     1381 taaatactag caaatgtcct aaatgaattt gtgttaatgt tgtctacaaa agaaaattag
     1441 cgttttactt gtacaactaa taataacttg gttattaaga gaatttcact tgttgactag
     1501 aaaaatcctt tcataatgaa acaattgcac cgataaattg tataaatata aaattaattc
     1561 taattgtttt tttttttcct gatcgatacc gtcgactcta gaggatcagc ttgggctgca
     1621 ggtcgaggga cctaataact tcgtatagca tacattatac gaagttatat taagggttcc
     1681 ggatcctcga gatggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca
     1741 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
     1801 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
     1861 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct
     1921 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca
     1981 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct
     2041 ccctacagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg
     2101 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc
     2161 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc
     2221 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
     2281 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg
     2341 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt
     2401 aactcgaggg atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact
     2461 acctacagag atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa
     2521 actactgatt ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga
     2581 gcagtggtgg aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag
     2641 tgatgatgag gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt
     2701 agaagacccc aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag
     2761 taatagaact cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata
     2821 caagaaaatt atggaaaaat attctgtaac ctttataagt aggcataaca gttataatca
     2881 taacatactg ttttttctta ctccacacag gcatagagtg tctgctatta ataactatgc
     2941 tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg gttaataagg aatatttgat
     3001 gtatagtgcc ttgactagag atcataatca gccataccac atttgtagag gttttacttg
     3061 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     3121 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     3181 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     3241 tatcttatca tgtctggatc tgacatggta agtaagctta tccccggagc ttgggctgca
     3301 ggtcgaggga cctaataact tcgtatagca tacattatac gaagttatat taagggttcc
     3361 ggatcgatcc cctcgacgga tccaccggtg gccggccagg cgcgccacca tgccagagcc
     3421 agcgaagtct gctcccgccc cgaaaaaggg ctccaagaag gcggtgacta aggcgcagaa
     3481 gaaagacggc aagagcgcag cgcaccgcaa ggagagctat tccatctatg tgtacaaggt
     3541 tctgaagcag gtccaccctg acaccggcat ttcgtccaag gccatgggca tcatgaattc
     3601 gtttgtgaac gacattttcg agcgcatcgc aggtgaggct tcccgccttg cgcattacaa
     3661 caagcgctcg accatcacct ccagggagat ccagacggcc gtgcgcctgc tgctgcctgg
     3721 ggagttggcc aagcacgccg tgtccgaggg tactaaggcc gtcaccaagt acaccagcgc
     3781 taagatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     3841 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     3901 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     3961 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     4021 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     4081 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     4141 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     4201 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     4261 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     4321 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     4381 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     4441 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     4501 gtaaagcggc cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt
     4561 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     4621 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     4681 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     4741 gtatcttaat cgaggggggg cccggtaccc accgcggtgg cggcgatctc tccccagcat
     4801 gcctgctatt gtcttcccaa tcctccccct tgctgtcctg ccccacccca ccccccagaa
     4861 tagaatgaca cctactcaga caatgcgatg caatttcctc attttattag gaaaggacag
     4921 tgggagtggc accttccagg gtcaaggaag gcacggggga ggggcaaaca acagatggct
     4981 ggcaactaga aggcacagtc gaggctgatc agcgggccgc tttacttgta cagctcgtcc
     5041 atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc
     5101 tcgttggggt ctttgctcag cttggactgg gtgctcaggt agtggttgtc gggcagcagc
     5161 acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgctgccg
     5221 tcctcgatgt tgtggcggat cttgaagttg gccttgatgc cgttcttctg cttgtcggcg
     5281 gtgatataga cgttgtcgct gatggcgttg tactccagct tgtgccccag gatgttgccg
     5341 tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac
     5401 ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg
     5461 acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag
     5521 cgggcgaagc actgcacgcc ccaggtcagg gtggtcacga gggtgggcca gggcacgggc
     5581 agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg
     5641 ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg
     5701 ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tgttgtcaga cctggtaact
     5761 ccttactgta acttattgca cctgatgaac aaaagatgtg aactgaacgc aagtaggagg
     5821 ctaatagagg ctagtacagt acaaagatcc ccaaatgatg tgtgccggcc ttagtggagg
     5881 cagacccctg gttatatacc tgtgctgcag aaagaggggc ttacaaatgg atctctctgg
     5941 aatggcatta tctcatggtg ggaacagtgt tgtcagcaga atcagcgggg tggactctga
     6001 cctgagaatg acagtctagc actgaatggc tcagacaata ctcttgtggc caactatcac
     6061 cggaaaataa gtgcctgcca aaacaaacca gaccagatga agctacatgg ctgtagaacg
     6121 tcagtattta atgtgttgtg gccacttggt tcaccagatt taagagctaa tttttttaaa
     6181 tatcacagat aatcaatcgc tttttttttt tcttgactga aaagcttaga ctggaaaatt
     6241 ctttgaaaaa gtttgcacta cagaaaataa atacataaca tcctgcactg aatgcacact
     6301 attaatggta cccagctttt gttcccttta gtgagggtta attgcgcgct agggataaca
     6361 gggtaatacg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     6421 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     6481 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggaaattgta
     6541 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aatcagctca ttttttaacc
     6601 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     6661 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     6721 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     6781 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     6841 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     6901 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     6961 cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa
     7021 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     7081 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     7141 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     7201 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     7261 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     7321 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     7381 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     7441 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     7501 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     7561 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     7621 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     7681 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     7741 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     7801 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     7861 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     7921 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     7981 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     8041 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     8101 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     8161 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     8221 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     8281 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     8341 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     8401 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     8461 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     8521 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     8581 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     8641 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     8701 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     8761 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     8821 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     8881 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     8941 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     9001 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     9061 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     9121 acacaggaaa cagctatgac catgattacg ccaagcgcgt
//
