LOCUS       sequence-11372-e        8199 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8199)
  AUTHORS   Potter CJ, Tasic B, Russler EV, Liang L, Luo L
  TITLE     The Q system: a repressible binary system for transgene expression, 
            lineage tracing, and mosaic analysis.
  JOURNAL   Cell. 2010 Apr 30. 141(3):536-48.
  PUBMED    20434990
REFERENCE   2  (bases 1 to 8199)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 30. 141(3):536-48."
COMMENT     Sequence Label: pCMV-QS
FEATURES             Location/Qualifiers
     source          1..8199
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3703..3732
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3748..3765
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3788..3805)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3794..4018
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4064..4492
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4151..4170)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4361..4382
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4501..4521)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4506..4835
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4686..4821
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4748..4767
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4902..5696
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4956..4975)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5566..5585
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5872..5993
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5909..5928)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5963..5982
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6042..6058)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6042..6058)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6055..6077)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6066..6082
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6090..6096,6097..6114,6115..6120))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6135..6156
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6273..6290)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6444..7032)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6524..6543)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7203..7994,7995..8063))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7826..7845
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8064..8168)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttggt acccaacatg aacaccatcc cggcacgcca tgtcggggat gtcgccgccc
      961 gcgatcctct acctctaccg cacatatcat cctccgtcgc cagcggcatg aagcgttcct
     1021 tcgcaaccat ggccatgctc tacaacgaca ctggcaacag caacgatgtc ggtgcccatg
     1081 ccaggcgacc accacgaacc ctctccaata gtcggagcac ttccgcccac agagtacctc
     1141 taggctcttg gtcggcgccc aattccccgc cccgccgtgc gctgccgcat catcccatca
     1201 ccgccagctt cgatcccgat gcttccattg ttatcgccgg cattcgtggc gctggcaagt
     1261 ctacgctggc catcatggca tctaccgcca tgaagcgcaa gatagtcgac ctggaatccg
     1321 agttccatca tcttactggc ttgtctagtt ccagctacaa gaagacacac ggcccggtcg
     1381 actatgggag gcgccagatc gccatcttgc agaacatctt gaatctgcac aggacccgcg
     1441 ccattctcgt ctgctcttgg ctggagcggg atgtgcaggc catgttgcag gatttcagcg
     1501 tgtctaatcc tgtcatttac gttctgcgcg atgccaaagc tatcgaggcc catctgaagg
     1561 gatacgacaa gtccaaagtt ggcaccctcc ttgatgccac cagtactgtc cttcgccgct
     1621 gcacccgttt cgagttcttc aacgtctccg aagagaacct ggacacccac tccgcttcaa
     1681 catcaccacc tgctgttccg gaccagcggc ataccgcgcc gtatctaacg cttaaacgag
     1741 ccgagcgcca cttcctcaaa tttctctcct tgattctacc caaggggacc ataccttttg
     1801 tcgagtccgc ctttcccctg gcttccgtcc ccgttgaaca gcgccgcttt acctacgccc
     1861 tcgccttgcc tgtatctgcc ttgctcgaca aaggcgtcga tatccaagag cttgatgtcg
     1921 gtgtagacgc aatcgagatc attgtagacg atcttgcaac gagcgaatcc ggcccaacga
     1981 gccccttggg tcttgcgccc caccgagcga gcgagatcag tcgtgttgta ggcgaaatca
     2041 ggagggacac agtgatcccc atcattctgc acgtggtctt tccagaaaga gcgctatatg
     2101 aagaagctct gctcgcgctt tacatgactt acctgaacca tgccttaagg cttgcgccag
     2161 attatcttac ggtcgatctg gggctcgatt ctggcttgct tgggcaacta accaccgttc
     2221 aaggaaccac caaggtcatc ggcaataaac aacttgcaga ggtcaattcg ccgcgctggg
     2281 gggatccatc ttggttacaa gcctatgaaa aggcccagaa tacaggatgc gacttggtaa
     2341 ggttgaccag accggcttca aatccccggg acaacacaga cattcggcag ttccacgttg
     2401 ctgtagaggc cgtcgggggt ccaaggctcc catttattgc ttacaacaca ggacgcctag
     2461 gtcggacatc gatgtgtttt aacgagatcc tgactccagt tacaccagtg cctaccaagg
     2521 aggatgcaat cgggctccgc aatccagccc atcgctatct ccagcctccg ctcacggctc
     2581 tggaagcaac acaggctctc tactcggcat ttgtccacga cccaatgaag ctgtatgtct
     2641 ttggcgcaaa tgtgggatat agcttgtccc cagccatgca caatgccgca ctcaaggcct
     2701 gtggcattcc acaccattac aagccccttt ccacagcaaa catcgggact ttgcgcgagg
     2761 ttatcagcga tccgcagttt gctggagcct cggtcggcct gccgtttaag gtggaaatca
     2821 tcagcctcac acactcgctg agccggcacg cgaaagccat cggagccgtc aacaccttga
     2881 ttccggtacg acaccttacc gcggacggtg gaataccgga cgaggtgtcc atgttcaaca
     2941 atatcagcca agccggcgct gtcagagctc tctacggcga gaacacggat tggattggta
     3001 tccgagcctg ccttcgccgc ggtttatcgc ccgccaatgc cgtgagatca acaagcactg
     3061 gtcttgtcat cggcgctggc ggaatggcta gggcagctgt ctatgccatg cttcaactgg
     3121 gagtcaagaa gattttgatc tttaaccgaa catttgctaa tgccgagaag ctggttctac
     3181 acttcgagaa cctgttggtc agagacgcat tgcctctgtt gagcacaggg ccaagatccc
     3241 acgacaacac ctgttttcac atcattcgat ctcgagacga tccgctccca gaaaacttca
     3301 aaaacccgac catgatcgtt tcctgcatac cgacacacac agtggacaac acccctgacc
     3361 ctgaatttac tgtgcctttg cactggctcg acaaccccac tggcggcatt gtactagaac
     3421 tcgactacaa atgtctcaca tcacccttgc tcgaacaaac acgacgcgag gctcacagag
     3481 gctgggtcgc aatggatgga cttgacctct tgccagaaca agggtttgcc caatttgaac
     3541 tgttcaccgg gcggcgagca cctcgtcgct tgatgaggcg cgaggttttg cgagcatacc
     3601 cagatgatca agcaaaatct cataccgcgc agttacagcc tcgcctcaac ggaattgcaa
     3661 cgcaaatatc ttgagcggcc gctcgagtct agagggccct tcgaacaaaa actcatctca
     3721 gaagaggatc tgaatatgca taccggtcat catcaccatc accattgagt ttaaacccgc
     3781 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     3841 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     3901 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     3961 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     4021 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     4081 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     4141 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     4201 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     4261 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     4321 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     4381 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     4441 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
     4501 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     4561 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     4621 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     4681 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     4741 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
     4801 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
     4861 ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
     4921 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     4981 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     5041 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
     5101 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     5161 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     5221 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     5281 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     5341 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     5401 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
     5461 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     5521 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     5581 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     5641 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     5701 gactctgggg ttcgcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     5761 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     5821 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     5881 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     5941 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     6001 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     6061 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     6121 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     6181 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     6241 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     6301 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     6361 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     6421 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     6481 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     6541 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     6601 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     6661 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     6721 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     6781 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     6841 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     6901 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     6961 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     7021 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     7081 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     7141 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     7201 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     7261 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     7321 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     7381 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     7441 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     7501 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     7561 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     7621 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     7681 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     7741 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     7801 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     7861 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     7921 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     7981 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     8041 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     8101 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     8161 gttccgcgca catttccccg aaaagtgcca cctgacgtc
//
