LOCUS       sequence-11382-e       14867 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14867)
  AUTHORS   Potter CJ, Tasic B, Russler EV, Liang L, Luo L
  TITLE     The Q system: a repressible binary system for transgene expression, 
            lineage tracing, and mosaic analysis.
  JOURNAL   Cell. 2010 Apr 30. 141(3):536-48.
  PUBMED    20434990
REFERENCE   2  (bases 1 to 14867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 30. 141(3):536-48."
COMMENT     Sequence Label: pmCD8-GFP,y+
FEATURES             Location/Qualifiers
     source          1..14867
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             789..1454
                     /codon_start=1
                     /product="mouse lymphocyte antigen CD8 alpha chain"
                     /label=mCD8
                     /translation="MASPLTRFLSLNLLLLGESIILGSGEAKPQAPELRIFPKKMDAEL
                     GQKVDLVCEVLGSVSQGCSWLFQNSSSKLPQPTFVVYMASSHNKITWDEKLNSSKLFSA
                     MRDTNNKYVLTLNKFSKENEGYYFCSVISNSVMYFSSVVPVLQKVNSTTTKPVLRTPSP
                     VHPTGTSQPQRPEDCRPRGSVKGTGLDFACDIYIWAPLAGICVALLLSLIITLICYHSR
                     "
     CDS             1461..2174
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(1491..1519)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2114..2134
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     intron          2261..2326
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2456..2476
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2748..2882
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2785..2804)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2839..2858
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             9389..9400
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(12621..12639)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12707..12811
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12812..12880,12881..13672)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMSVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13030..13049)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13843..14431
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14332..14351
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    join(14633..14834,14835..14865)
                     /label=P element 3' end
                     /note="P element 3' end"
ORIGIN
        1 cgttcccatg tgttggtctg tatgccgtag tgctgccgtc gcagcggttt tcacgccaaa
       61 tctggcttgt ttcagaggtc aggaactaac ttagtctgca ggtgtagtta gcatgttccc
      121 gcctacacag gttcactgaa caaaagtatt taaacataaa aatatctatt ttatagatac
      181 aatttttctt gttcttatag tttttactaa gaaaacgatt agttataaaa aatattatgt
      241 aagtgatgtc aaaaaagaaa gtagcatgtc gttggtattc ttttcataat tgagttcaag
      301 gctaaacttt ttctattcca tatattttta aataatttat atcattaccc gaattatatt
      361 tcaaaggaaa aacagttagg taaactttct tctattatat ttttactctt taaaaacttt
      421 cctatgcatt gtagtaacgt tttagatttt tttttgccta tttatttaaa cataaatcag
      481 caaattctat tttattcata aatgtcccac caaccaatgt tcttcaagac aatagcctac
      541 agcactagtg ccccagttcg ggcaaggtca tcctggagac gcttaacttc tccgccgccg
      601 atctgccgct ggactacgtg ggtctggccc atgatgaaat aacataaggt ggtcccgtcg
      661 atagccgaag cttaccgaag tatacactta aattcagtgc acgtttgctt gttgagagga
      721 aaggttgtgt gcggacgaat ttttttttga aaacattaac ccttacgtgc ggccgcggct
      781 cgagcaaaat ggcctcaccg ttgacccgct ttctgtcgct gaacctgctg ctgctgggtg
      841 agtcgattat cctggggagt ggagaagcta agccacaggc acccgaactc cgaatctttc
      901 caaagaaaat ggacgccgaa cttggtcaga aggtggacct ggtatgtgaa gtgttggggt
      961 ccgtttcgca aggatgctct tggctcttcc agaactccag ctccaaactc ccccagccca
     1021 ccttcgttgt ctatatggct tcatcccaca acaagataac gtgggacgag aagctgaatt
     1081 cgtcgaaact gttttctgcc atgagggaca cgaataataa gtacgttctc accctgaaca
     1141 agttcagcaa ggaaaacgaa ggctactatt tctgctcagt catcagcaac tcggtgatgt
     1201 acttcagttc tgtcgtgcca gtccttcaga aagtgaactc tactactacc aagccagtgc
     1261 tgcgaactcc ctcacctgtg caccctaccg ggacatctca gccccagaga ccagaagatt
     1321 gtcggccccg tggctcagtg aaggggaccg gattggactt cgcctgtgat atttacatct
     1381 gggcaccctt ggccggaatc tgcgtggccc ttctgctgtc cttgatcatc actctcatct
     1441 gctaccacag ccgcggatcc atgagtaaag gagaagaact tttcactgga gttgtcccaa
     1501 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
     1561 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac
     1621 ctgttccatg gccaacactt gtcactactt taacttatgg tgttcaatgc ttttcaagat
     1681 acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtcc
     1741 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt
     1801 ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg
     1861 gaaacattct tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg
     1921 cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg
     1981 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc
     2041 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa
     2101 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg
     2161 atgaactata caaataatct agaggatctt tgtgaaggaa ccttacttct gtggtgtgac
     2221 ataattggac aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag
     2281 tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg
     2341 aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga
     2401 agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa
     2461 aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag
     2521 tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa
     2581 agctgcactg ctatacaaga aaattatgga aaaatatttg atgtatagtg ccttgactag
     2641 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     2701 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     2761 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     2821 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     2881 tccactagaa ggccttagta tggggtaccc caagggcgaa ttctgcagat atccatcaca
     2941 ctggcggccg cagcttgatt cgactattaa atgattatcg cccgattacc acattgagtg
     3001 gtttaaaata gccataaaat atgcaactga cgatggctta agataaatac gtcgcagagt
     3061 cactcataaa tttcgaacgc agcccgctga tttacctacc cctctaaacg attcatagta
     3121 tatgtacgag tatatccact aagctttttc gagcactgat tttttcgctt gcacgagaca
     3181 agtgcaccac cgcaattgca ggcaaattat gtctgaggta atgattccgt ttcgtgcaag
     3241 attacacaga aatcaaatta cgacaacctt tattcagtaa gcaaacaaag cctttgttgg
     3301 catctaatta ttccacttat ggttgcgatt tcgggagcta caatcggttt tggtttagta
     3361 tatctagcga gttccttggc gacatttaaa atttacaaat aaagtttctc tattcaatcg
     3421 ggacagtgga aattgactat tttatttata ttaatgaact tatttttaat ttggcttaag
     3481 ttactaaggg gtactaatag tttgagcgca gtgcatgtca tggggacatg tgcaattgtg
     3541 tgtaagcggg aagtgatcgc ggccttccga atttggccat gccaaataat cccagctcga
     3601 aaggagggga cccggcggtc agggccatgg acattgaact tgaaaaaaaa aaaaacacaa
     3661 aaatatataa cacaaaacgg aaaatgctgt gtaccgctta tgttagagaa gttgagcaac
     3721 gggtttttcg ttttgcagtc acgatggatt tccaaattag tgtaggaggg gggaggggag
     3781 ggagggagat aatgtccagg ctgccataag tggggaataa ggaaaataaa acatgaaaca
     3841 cgggtcgggc aatgtcatgc ggtattcggc tttgctttcc gcccaagttg aagtgatcct
     3901 gtgtgtaaat aatgtcgaat gttgccggtc ggttgcataa gcgttagtca attatgccaa
     3961 agagatctga tttgtggaag ctttttttga ccacttagcg cgctccgctt gatgttgttt
     4021 tgttttgtgc tggggcagaa aacttgtttc aattattggg aaaagtgcgt ataaatcatt
     4081 gccgcaagct ctgaaaagcg aaaaagaaaa acagtaacca aacagacaaa cgcagcatcc
     4141 ccccacacaa ttaagcaaaa acttgaaaac aagtcaattc gaaaaaaatt ataggttcaa
     4201 cggctgcagc gatcgcatca ttagttgcgt ttttagtaaa tacaccattt cattacacaa
     4261 cacacacaat taattaataa aactgtactg gttatttcaa gtgtgtcttt taataagcct
     4321 gccgatcgca ataaattcga gcagcattgc cggtaatttt gtgcaacata tttttcgatt
     4381 gccacaccgt gtttgtttat ttttctgtgg gtgcaatgat ttagaatgcg ggcaagggat
     4441 caagttgaac cacttctaag aaaaaatagc attgcataaa tgatatagag tccaaaaact
     4501 acacaaattc aatagcagta atggttacat tagctttgaa attgttttta gacatccgaa
     4561 gaaataagat taaatttaaa cggcattctt taatttgtat tttaatattt tgagaggttt
     4621 tccttattta aagtgtagat tattgaggat taatgcaaac cactttatct gcggaggtcg
     4681 taaaacgtat ttttacccat ttgcatgttt attatgcgtg tggctggttg tattacttta
     4741 cttaagtttt gcaatttttt ctttagcaag caggtgcatt tgggccaaga gatatatgcg
     4801 atcgctttcg gttcgaattt ttaacattta cttgcggcga tggtcattag agcattaccc
     4861 acttagggca cccccaacat ccagttgatt ttcagggacc acaatatttt aaataacagc
     4921 tagtggaatt acctaaaagc gctttcgtcc cttttgaaat tttatgtaac actcaattat
     4981 atttatgtat atgtatgctc aaaatcacct gccaataact agcggaaacc aaatatttga
     5041 ccctcagtga attgtgaatc atcggtgacg cccaatcgaa atccaatcct aagcaattga
     5101 aacgagcacg agttccaatt taatagtata caaggaaaca cctgctttaa atactctaca
     5161 tagtacacgt tataataacg atttatttga tatttctgga tttttgtctg catgtatttc
     5221 atataatatt gatttgattt ttttaatgaa ttgaactaaa aaatcatatt agaacatttt
     5281 ttgcagtcgc cgataaagat gaacactgtt ctcagaacac aactgtcatg tattaagctt
     5341 tcagattttc agaaatttgg agagcaatgc attctatgca cgagcctcct ggccttacaa
     5401 tttacttgtt tgaaattaga tcgtcaaata aagtccctaa aattaaataa atagtagtca
     5461 caactttaaa ataggtctta atcttttagg gtaccgaaag gtatttcggc acaaatcagc
     5521 gcagttttaa atgtcgatga aggccaaaaa tcataccaaa cccagcgaaa ggtgatgtct
     5581 gactcattaa attgggggat tcgagtgtat ttattaaaca tgcgtgaaaa tcaatcatgg
     5641 aagacaaaac gcaaagttgg ccgatctatg ggaacagcat aagccacctg attacccgaa
     5701 cactgaacca cccgaatcac taaaaccacc gaagttggcg cgcgccttcg ttttcatttt
     5761 cattggcctg tcttcgtctt cggagaaaaa aaacttcata taaaacgcgg ccgacatatt
     5821 atggccacca gtcgttaccg cgccacggtc cacagaagag gattaaaaaa atatcacaca
     5881 gccgaaggct agagaagaac cccctatagc tgaacatata taaacaaata tatttttttt
     5941 tattgccaac acactttggc ttaagtgtta agagtgattg tcagcttaga gctaagtgca
     6001 ctgttccagg acaaagggtg gatccttgtg accctgatca ccttggtgac gccgtcttgg
     6061 gctgcttaca aacttcagga gcgatatagt tggagccagc tggactttgc tttcccgaat
     6121 acccgactaa aggaccaagc tctggctagt ggagattata ttccgcaaaa tgctctacct
     6181 gttggagtcg aacactttgg caatcggtta ttcgtcactg ttccccgctg gcgtgatggt
     6241 aagtggaagt taaatatgaa gcccttgggg agatcgtaaa tgggacattc ttacttaggg
     6301 catcagagat atctgattga gtggttgaca gttttatatg gcttgtttga catgatgtaa
     6361 aaacacaaaa ttcatttagt ttaggtattc aaataagagc ttgttattta ttttagaatt
     6421 tggagaacat ttttttgtct ttctaccctt cttagaaaat aatattgttt tgtacaattt
     6481 aattttaaac tagtacagac gaaaaatgta tttttttatt tgtatgcctt ttcaccattt
     6541 tggcagagga ataaatatga caatatattt tgagagcacc ctcatgtaaa ggttttagcg
     6601 tggcgacctc tcataaatcc ggttggtacc tgcgcgttat tttaacattt taaacaatta
     6661 accgttgtaa aatcgaagcc aatagcatgg cattggcttt atactgtatt aaattgtatt
     6721 atattaccat ccgaattgta aagacttctt cagggccgcc acatagaaat ggaaatccaa
     6781 tcacaaacaa taacttatgg cattagctat taaccgacga ttagctgtca gttcaacaaa
     6841 tgtaagagtg gcgaaatgtt taaatgcgaa ggcattgttc tgtgactcac gttttattat
     6901 taatcacaca aatgattttg cttcaaaatt atttggctta cacaataatc aaaattttta
     6961 tgaaatagtt gaaacacaaa actaggaaat tttaaaaagc aatgaaacta aaaaacccaa
     7021 ttgctaagat tatatgatgc gcatacaaat acttcagtac gtctaggaat gctttcgatg
     7081 attgattagt ttttatgcat ggcttacaat tggtatttac acagaaaaac acggcgatgt
     7141 taataaattt tgtacatatg ttcttaagca gtccgaaaca cccaaacttc tgactaaact
     7201 taaaaaaaca cgctcttcaa ggatatctta atgtcactta taatgtaatg gtattgtatt
     7261 attaagtata tcaaaatatt ctggcccagc cttgaggtct ctttttaaaa agatatcgac
     7321 tgactacctc cagtcaatga aataatagcc cagaaggccg aatcggcaaa aaataaaccc
     7381 caagttacgg caaacaaaac atagtgaaag ttgtggcaaa gtggaacatt taaaggcatg
     7441 cttcaatggc catcgaagca aatcaattag tcaaagcaaa tcggtagtgg caacaacagg
     7501 ctacagaata cctataagtg acagttatgg ggtatgatta attataaata ttatcattga
     7561 ccaccaatgc tgggctcaat tggaaaaact attctatgaa gatttgagta aataaatttt
     7621 gatttaaaaa agcccatggt tatcgcgaca actagctacg ggacaagatt actgtttaaa
     7681 atcaagtgtg aaatatcaaa atcaaaatcg gattccgatc gggaagttgt atccgattct
     7741 gaaactaaaa cacagaattg ccaacatttt ccgatatcga ctcagctcac gtatttcata
     7801 cagattcatt aggccaccag ccattgaata atatacccca gtcaattgag ctactcgata
     7861 gttgatcaac ttagcttttg tcaacgagtg aacgcataaa ctactacatc aacgatattt
     7921 gcggcccatt ccaagctaaa agttcatctt aattacaaat aagattagaa aaaatatctg
     7981 aatgaaaaaa atgttgagac atatttcttt ggaaaaggag aacctcaaga cagtcgaaaa
     8041 aattgtttac aatgaaaatg ttgaaaatca tgaagcagat aaatctgtca gttgcgaggt
     8101 tttaggactg aaagagcaca tgtcaaaata taaatttgtt caaatacttt atatttgact
     8161 gaattagatt gttattttaa aagttatgaa ttaaataaag attgaaaggt gcattatgct
     8221 caaatgtata tttatcgcaa cccccggtta ctttgtaaag caaaaacgcc tggtttgatt
     8281 tttaagaaga tgggtcggta aatcgataaa agctatattt tctggtcgtt gcagtctcac
     8341 tcgcctgcta taaaaacatt aaaagttccc agaaacaata aatgtcttta aattcaatta
     8401 acgaagaaat aaagaaggaa aagaactgga gcggaaatcg gtcgaaatac tgccaatggc
     8461 cacatataca tttaacagcg atatatggta tacatattga taatgatgtc agacgcaatt
     8521 gcttcagacg gctaatgaca tcgcaaattg cacgcaactt gcaatagtgc caattatgac
     8581 tgaagtacat atagccgggg atcttttaac ataaacttcc agtagatgta caagcagaaa
     8641 aaagagccat tagcacggca gttaccattg cttatgattc cttgtgtcca aaataatgac
     8701 aaataggtat ataaataatt aaatgccaaa cataagcgat tctaatttac ctttacatct
     8761 gtatgcattt acatattatc cagaaaacag acagcgataa cttgcaacat tgcttagtat
     8821 aataatccaa agaaggaatt taggcagaaa ttccagttaa ttaaatattc aaaacaaact
     8881 ttatttagtg cctcaataat agtttggccc tgctaattct cctattttat tttttaggga
     8941 ttccggccac tctgacctat ataaacatgg accgcagttt gacgggttca ccggagctaa
     9001 ttccgtatcc agattggcgc tcaaatacag ctggagattg cgccaacagt attaccactg
     9061 cctaccgcat taaagtggat gagtgtggtc ggctgtgggt tttggacact ggaaccgtgg
     9121 gcatcggcaa taccaccact aatccgtgcc cctatgcggt aaatgtcttt gacttgacca
     9181 cggatacgcg aattcggaga tacgagctac ctggcgtgga cacaaatcca aatactttca
     9241 tagctaacat tgccgtggat ataggcaaaa attgcgatga tgcatatgcc tattttgccg
     9301 atgaattggg atacggcttg attgcttact cctgggaact gaacaagtcc tggagattct
     9361 cggcacattc gtattttttc cccgatccat tgaggggcga tttcaatgtc gctggtatta
     9421 acttccaatg gggcgaggag ggtatatttg gtatgtccct ttcgcccatt cgatcggatg
     9481 gttatcgtac cctgtacttt agtccgttag caagtcatcg acaatttgcc gtatccacga
     9541 ggattttgag ggatgaaacc aggacggaag atagctatca tgactttgtt gccttagatg
     9601 aacggggtcc aaactcccat accacttcac gtgtgatgag cgatgatgga attgagctgt
     9661 tcaatttaat agatcaaaat gcagtgggtt gctggcactc atcaatgccg tactcaccgc
     9721 aatttcatgg cattgtggat cgcgatgacg ttggcttagt ttttccggcc gatgtgaaaa
     9781 ttgatgagaa caaaaacgtt tgggttctat ccgataggat gcccgttttc ttgctgtctg
     9841 acttggatta ttcagatact aatttccgaa tttacacggc tcccttggcc actttaattg
     9901 agaatactgt gtgtgatttg aggaataacg cctatgggcc gccaaatacc gtttcaatac
     9961 caaaacaagc cgttttgcca atgggtccac cgttatatac gaaacaatat cgtcctgtct
    10021 tgccacagaa acctcagacc agctgggctt cctcgccgcc tcctccaagt cgcacttatt
    10081 tgcccgccaa ttcaggcaat gtagtctcca gtattagtgt ctctacaaat tctgtgggtc
    10141 ctgcaggagt ggaggtgcca aaggcctata ttttcaacca gcacaacggc ataaattacg
    10201 agacaagtgg tccccatcta tttcccaccc atcaacccgc ccaaccgggt ggccaggatg
    10261 gtgggttaaa aacttatgtg aatgcccgcc aatctgggtg gtggcatcat cagcatcaag
    10321 gttaacataa tcctacacac ggtacttggg tatattctca cacactcgat tgatgtaaag
    10381 aatatttaaa gacaacaaca tagggcaaca gcggttaaaa aaaccacatg acgtatgagc
    10441 aagtggcaaa tcaatacttt atctagttat gttaagcaaa aaataacaat aaatcaactt
    10501 ttttttgaag gttaagagtt tacgcaattt tcttgagcgg aaaaagcgga aaaaatgtaa
    10561 gtatgcataa attctaaata tatcaacaac tgtacatttt ctggagtact actaccaggc
    10621 aagaaagtag gttgataaag ctatgcacaa gatctgtcat atggatgggt ttcgcatctt
    10681 ctggatcata ggacttgaga acatacacaa acacaactaa aatcagaaaa ggttgccagc
    10741 aatcacttta gttttctgca agctagatcc acctgctact atccctactt tcttctgttg
    10801 gggttgtcag agctctacct acagttgatt aaaccacaca tcagtcatcg tgtttttttt
    10861 acaattttcc taatttcctt acttgtattg cctacttttt tgcgagggac ttctattggc
    10921 cagtatatat tatgtgttgc atactcttgg tcacagcaaa aataaaacaa aaaatacgca
    10981 tggtaaaaga atgcttcctt ttttacgcac aacggtagac gaagcatata atttttacat
    11041 atcggaaatg ttcttgaaac agagcttagt aaatttatac gcaatattag ttagtgtatt
    11101 ttaattttcg ccttttagtt tcaccttgtt gtgtaatcag ggaaagtgat aatgtattta
    11161 ataataatta atagttttaa tgatacaaat ctattgcgca gaaatccgat gggtattaca
    11221 acattatgat tccttctaca ttatagatac atgcaaattg tatatctcga ctatagcgtt
    11281 ctctcttgtt tatgctacaa atgtaaatgt actaaagaaa atattttata aagtataaat
    11341 ttaatatatt atgacatttg atatatgtca tttataacgt ggaaagaata tttattatac
    11401 tttcgaaagt atcaaattag taagcacttt tttaaaaatc ataattaaca attaacaatt
    11461 ccaataaaaa ttgtttttgg gtgcatatta gtttgaagaa tcaaacttta aaatatgagt
    11521 ttgaaattgc agcatacata catacacata aggcaaaatt tagagctttt ttttcggtgc
    11581 atcggcaatt atttatttac actaccaggc gactgttgat attcccagtc tcgggcgggg
    11641 cattttgtca aactgtcgaa aaacaaacag aggaacaaag agcaggcagc atttatatac
    11701 tttgtgatta cggacggaca gtcccaaaaa gcattctgtg cgaagcactt aacgccaaaa
    11761 gtgaacaaaa caacacttgc tctattaaca cgccacactg tctgaaggca gtcggcttaa
    11821 cgagaaacaa gaatatccta cgtgtggcta atttgcggtt aaggaaggcc ccatgatata
    11881 taaagccaaa aaataaagca aaaccgcagc agttcattca aattttttgt ggccaaaaca
    11941 aatattaaga taaaagtcag tggggactat aatttgagtt attcaaatgc aataaatccg
    12001 cggaaccaaa cgagttgcta attttacgtt agacgccctt taatttcacg ctgccatttg
    12061 tggcttccgt ttcgtataag tttgggtcga tgatttttag aatttgcatt ttcaatttga
    12121 atataaaaaa ccgccgctgc aggtttgcag cttttaaagt gatccgcaat ctcattccta
    12181 gtcagtcgaa aaaaggtaag atagagaaaa gaacagaaat gactacaaaa ttaagtaccg
    12241 aattttgtgc catcgtaata gtttttcgcc atttcccttt cacgggttta actgttaatt
    12301 atgtaattaa atgtaaagtc gtggaactcg gcgttttttc ttgatcaata tacacatatt
    12361 tccggcgcaa atgctatttt tatgagcatt tcatgtaatt attgagacta ataggaagac
    12421 agatcttgtt tgggtgcagg gaaagttcaa cttaatcgct caatttgaga tcgcctggtc
    12481 ccttgagatt cgactgtaat tgaaattttt gcttttgatc ggagccagac ttcagacggg
    12541 gcaaacaaaa agactttgtt ggtggtaggg taggatccgt tgacctgcag gtcgaggaat
    12601 taattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
    12661 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
    12721 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
    12781 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
    12841 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
    12901 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
    12961 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
    13021 taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg
    13081 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
    13141 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
    13201 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
    13261 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
    13321 cataccaaac gacgagcgtg acaccacgat gtctgtagca atggcaacaa cgttgcgcaa
    13381 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
    13441 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
    13501 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
    13561 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
    13621 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
    13681 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
    13741 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
    13801 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
    13861 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
    13921 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
    13981 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
    14041 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
    14101 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
    14161 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
    14221 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
    14281 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
    14341 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
    14401 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcaagct tctagagatc
    14461 tgcaggtcga cggatccgtc gagcgtataa ccatctgtac aaaaaaggat ttcctttgcc
    14521 cagtcgtacg actttgtaca gatggttatc agatgtggac ataaaaagag gatgtttgga
    14581 tgtggtcata gacctaatgg acagtgatgg agttgatgac gccgacaagc tttgcgtact
    14641 cgcaaattat taaaaataaa actttaaaaa taatttcgtc taattaatat tatgagttaa
    14701 ttcaaacccc acggacatgc taagggttaa tcaacaatca tatcgctgtc tcactcagac
    14761 tcaatacgac actcagaata ctattccttt cactcgcact tattgcaagc atacgttaag
    14821 tggatgtctc ttgccgacgg gaccacctta tgttatttca tcatggg
//
