LOCUS       sequence-113847-         319 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses FokI-dCas9 and gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Nakagawa Y, Sakuma T, Sakamoto T, Ohmuraya M, Nakagata N, Yamamoto T
  TITLE     Production of knockout mice by DNA microinjection of various 
            CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes.
  JOURNAL   BMC Biotechnol. 2015 May 22;15(1):33.
  PUBMED    25997509
REFERENCE   2  (bases 1 to 319)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2015-05-22"; volume: "15(1)"; pages: "33"
COMMENT     Sequence Label: pX330A_FokI-1x2
FEATURES             Location/Qualifiers
     source          1..319
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(46..68)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    57..73
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(81..87,88..105,106..111))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    126..147
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tgagggttaa ttgcgcgctt ggcgtaatca tggtcatanc tgtttcctgt gtgaaattgt
       61 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
      121 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgccagc tttccaccgg
      181 tggtctcttc tagaggtacc cgttacataa cttacggtaa atggcccgcc tggctgaccg
      241 cccaacgacc cccgcccatt gacntcaata gtaacgccaa tagggacttt ccattgacgt
      301 caatgggtgg agtatttac
//
