LOCUS       sequence-11389-e       10238 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10238)
  AUTHORS   Potter CJ, Tasic B, Russler EV, Liang L, Luo L
  TITLE     The Q system: a repressible binary system for transgene expression, 
            lineage tracing, and mosaic analysis.
  JOURNAL   Cell. 2010 Apr 30. 141(3):536-48.
  PUBMED    20434990
REFERENCE   2  (bases 1 to 10238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 30. 141(3):536-48."
COMMENT     Sequence Label: pQUAST-FLPo
FEATURES             Location/Qualifiers
     source          1..10238
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(315..334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(994..1785,1786..1854))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1617..1636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1855..1959)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2027..2045
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2083..2105)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2205..2224
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(2761..2791,2792..2993)
                     /label=P element 3' end
                     /note="P element 3' end"
     promoter        3189..3427
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     3374..3395
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          4867..4932
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5062..5082
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5354..5488
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5391..5410)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5445..5464
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5515..5533)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(5993..6064,6459..6732,6807..7461,7523..7838,8059..8190,
                     8261..8875)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(9653..10207,10208..10238)
                     /label=P element 5' end
ORIGIN
        1 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
       61 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
      121 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
      181 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2221 tgtactgaga gtgcaccata tgcggtgtga aataccgcac cgaatcgcgc ggaactaacg
     2281 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     2341 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     2401 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     2461 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     2521 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     2581 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     2641 atggtaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
     2701 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
     2761 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
     2821 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
     2881 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
     2941 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
     3001 gctgcatcca acgcgttggg agctctccgg atccaagctt ggatccgggt aatcgcttat
     3061 cctcggataa acaattatcc tcacgggtaa tcgcttatcc gctcgggtaa tcgcttatcc
     3121 tcgggtaatc gcttatcctt aagcttgata tcgaattcct gcagcccgag cggagactct
     3181 agcgagcgcc ggagtataaa tagaggcgct tcgtctacgg agcgacaatt caattcaaac
     3241 aagcaaagtg aacacgtcgc taagcgaaag ctaagcaaat aaacaagcgc agctgaacaa
     3301 gctaaacaat ctgcagtaaa gtgcaagtta aagtgaatca attaaaagta accagcaacc
     3361 aagtaaatca actgcaacta ctgaaatctg ccaagaagta attattgaat acaagaagag
     3421 aactctgaat agggaattgg gaattcgtta acagatcggg ggatcaattc gttaacagat
     3481 ctcaacatga gccagttcga catcctgtgc aagacccccc ccaaggtgct ggtgcggcag
     3541 ttcgtggaga gattcgagag gcccagcggc gagaagatcg ccagctgtgc cgccgagctg
     3601 acctacctgt gctggatgat cacccacaac ggcaccgcca tcaagagggc caccttcatg
     3661 agctacaaca ccatcatcag caacagcctg agcttcgaca tcgtgaacaa gagcctgcag
     3721 ttcaagtaca agacccagaa ggccaccatc ctggaggcca gcctgaagaa gctgatcccc
     3781 gcctgggagt tcaccatcat cccttacaac ggccagaagc accagagcga catcaccgac
     3841 atcgtgtcca gcctgcagct gcagttcgag agcagcgagg aggccgacaa gggcaacagc
     3901 cacagcaaga agatgctgaa ggccctgctg tccgagggcg agagcatctg ggagatcacc
     3961 gagaagatcc tgaacagctt cgagtacacc agcaggttca ccaagaccaa gaccctgtac
     4021 cagttcctgt tcctggccac attcatcaac tgcggcaggt tcagcgacat caagaacgtg
     4081 gaccccaaga gcttcaagct ggtgcagaac aagtacctgg gcgtgatcat tcagtgcctg
     4141 gtgaccgaga ccaagacaag cgtgtccagg cacatctact ttttcagcgc cagaggcagg
     4201 atcgaccccc tggtgtacct ggacgagttc ctgaggaaca gcgagcccgt gctgaagaga
     4261 gtgaacagga ccggcaacag cagcagcaac aagcaggagt accagctgct gaaggacaac
     4321 ctggtgcgca gctacaacaa ggccctgaag aagaacgccc cctaccccat cttcgctatc
     4381 aagaacggcc ctaagagcca catcggcagg cacctgatga ccagctttct gagcatgaag
     4441 ggcctgaccg agctgacaaa cgtggtgggc aactggagcg acaagagggc ctccgccgtg
     4501 gccaggacca cctacaccca ccagatcacc gccatccccg accactactt cgccctggtg
     4561 tccaggtact acgcctacga ccccatcagc aaggagatga tcgccctgaa ggacgagacc
     4621 aaccccatcg aggagtggca gcacatcgag cagctgaagg gcagcgccga gggcagcatc
     4681 agataccccg cctggaacgg catcatcagc caggaggtgc tggactacct gagcagctac
     4741 atcaacaggc ggatctgata agcggccgcg gctcgagggt acctctagag gatctttgtg
     4801 aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc
     4861 tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt
     4921 tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg gaatgccttt
     4981 aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga ggctactgct
     5041 gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc caaggacttt
     5101 ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac tcttgcttgc
     5161 tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat tatggaaaaa
     5221 tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt
     5281 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     5341 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     5401 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     5461 catcaatgta tcttatcatg tctggatcgg atccactaga aggccttagt atgtatgtaa
     5521 gttaataaaa cccttttttg gagaatgtag atttaaaaaa acatattttt tttttatttt
     5581 ttactgcact ggacatcatt gaacttatct gatcagtttt aaatttactt cgatccaagg
     5641 gtatttgaag taccaggttc tttcgattac ctctcactca aaatgacatt ccactcaaag
     5701 tcagcgctgt ttgcctcctt ctctgtccac agaaatatcg ccgtctcttt cgccgctgcg
     5761 tccgctatct ctttcgccac cgtttgtagc gttacctagc gtcaatgtcc gccttcagtt
     5821 gcactttgtc agcggtttcg tgacgaagct ccaagcggtt tacgccatca attaaacaca
     5881 aagtgctgtg ccaaaactcc tctcgcttct tatttttgtt tgttttttga gtgattgggg
     5941 tggtgattgg ttttgggtgg gtaagcaggg gaaagtgtga aaaatcccgg caatgggcca
     6001 agaggatcag gagctattaa ttcgcggagg cagcaaacac ccatctgccg agcatctgaa
     6061 caatgtgagt agtacatgtg catacatctt aagttcactt gatctatagg aactgcgatt
     6121 gcaacatcaa attgtctgcg gcgtgagaac tgcgacccac aaaaatccca aaccgcaatc
     6181 gcacaaacaa atagtgacac gaaacagatt attctggtag ctgtgctcgc tatataagac
     6241 aatttttaag atcatatcat gatcaagaca tctaaaggca ttcattttcg actacattct
     6301 tttttacaaa aaatataaca accagatatt ttaagctgat cctagatgca caaaaaataa
     6361 ataaaagtat aaacctactt cgtaggatac ttcgttttgt tcggggttag atgagcataa
     6421 cgcttgtagt tgatatttga gatcccctat cattgcaggg tgacagcgga gcggcttcgc
     6481 agagctgcat taaccagggc ttcgggcagg ccaaaaacta cggcacgctc ctgccaccca
     6541 gtccgccgga ggactccggt tcagggagcg gccaactagc cgagaacctc acctatgcct
     6601 ggcacaatat ggacatcttt ggggcggtca atcagccggg ctccggatgg cggcagctgg
     6661 tcaaccggac acgcggacta ttctgcaacg agcgacacat accggcgccc aggaaacatt
     6721 tgctcaagaa cggtgagttt ctattcgcag tcggctgatc tgtgtgaaat cttaataaag
     6781 ggtccaatta ccaatttgaa actcagtttg cggcgtggcc tatccgggcg aacttttggc
     6841 cgtgatgggc agttccggtg ccggaaagac gaccctgctg aatgcccttg cctttcgatc
     6901 gccgcagggc atccaagtat cgccatccgg gatgcgactg ctcaatggcc aacctgtgga
     6961 cgccaaggag atgcaggcca ggtgcgccta tgtccagcag gatgacctct ttatcggctc
     7021 cctaacggcc agggaacacc tgattttcca ggccatggtg cggatgccac gacatctgac
     7081 ctatcggcag cgagtggccc gcgtggatca ggtgatccag gagctttcgc tcagcaaatg
     7141 tcagcacacg atcatcggtg tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa
     7201 gcgtctggca ttcgcctccg aggcactaac cgatccgccg cttctgatct gcgatgagcc
     7261 cacctccgga ctggactcat ttaccgccca cagcgtcgtc caggtgctga agaagctgtc
     7321 gcagaagggc aagaccgtca tcctgaccat tcatcagccg tcttccgagc tgtttgagct
     7381 ctttgacaag atccttctga tggccgaggg cagggtagct ttcttgggca ctcccagcga
     7441 agccgtcgac ttcttttcct agtgagttcg atgtgtttat taagggtatc tagcattaca
     7501 ttacatctca actcctatcc agcgtgggtg cccagtgtcc taccaactac aatccggcgg
     7561 acttttacgt acaggtgttg gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga
     7621 tcgccaagat atgcgacaat tttgctatta gcaaagtagc ccgggatatg gagcagttgt
     7681 tggccaccaa aaatttggag aagccactgg agcagccgga gaatgggtac acctacaagg
     7741 ccacctggtt catgcagttc cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg
     7801 aaccactcct cgtaaaagtg cgacttattc agacaacggt gagtggttcc agtggaaaca
     7861 aatgatataa cgcttacaat tcttggaaac aaattcgcta gattttagtt agaattgcct
     7921 gattccacac ccttcttagt ttttttcaat gagatgtata gtttatagtt ttgcagaaaa
     7981 taaataaatt tcatttaact cgcgaacatg ttgaagatat gaatattaat gagatgcgag
     8041 taacatttta atttgcagat ggttgccatc ttgattggcc tcatcttttt gggccaacaa
     8101 ctcacgcaag tgggcgtgat gaatatcaac ggagccatct tcctcttcct gaccaacatg
     8161 acctttcaaa acgtctttgc cacgataaat gtaagtcttg tttagaatac atttgcatat
     8221 taataattta ctaactttct aatgaatcga ttcgatttag gtgttcacct cagagctgcc
     8281 agtttttatg agggaggccc gaagtcgact ttatcgctgt gacacatact ttctgggcaa
     8341 aacgattgcc gaattaccgc tttttctcac agtgccactg gtcttcacgg cgattgccta
     8401 tccgatgatc ggactgcggg ccggagtgct gcacttcttc aactgcctgg cgctggtcac
     8461 tctggtggcc aatgtgtcaa cgtccttcgg atatctaata tcctgcgcca gctcctcgac
     8521 ctcgatggcg ctgtctgtgg gtccgccggt tatcatacca ttcctgctct ttggcggctt
     8581 cttcttgaac tcgggctcgg tgccagtata cctcaaatgg ttgtcgtacc tctcatggtt
     8641 ccgttacgcc aacgagggtc tgctgattaa ccaatgggcg gacgtggagc cgggcgaaat
     8701 tagctgcaca tcgtcgaaca ccacgtgccc cagttcgggc aaggtcatcc tggagacgct
     8761 taacttctcc gccgccgatc tgccgctgga ctacgtgggt ctggccattc tcatcgtgag
     8821 cttccgggtg ctcgcatatc tggctctaag acttcgggcc cgacgcaagg agtagccgac
     8881 atatatccga aataactgct tgtttttttt tttaccatta ttaccatcgt gtttactgtt
     8941 tattgccccc tcaaaaagct aatgtaatta tatttgtgcc aataaaaaca agatatgacc
     9001 tatagaatac aagtatttcc ccttcgaaca tccccacaag tagactttgg atttgtcttc
     9061 taaccaaaag acttacacac ctgcatacct tacatcaaaa actcgtttat cgctacataa
     9121 aacaccggga tatatttttt atatacatac ttttcaaatc gcgcgccctc ttcataattc
     9181 acctccacca caccacgttt cgtagttgct ctttcgctgt ctcccacccg ctctccgcaa
     9241 cacattcacc ttttgttcga cgaccttgga gcgactgtcg ttagttccgc gcgattcggt
     9301 tcgctcaaat ggttccgagt ggttcatttc gtctcaatag aaattagtaa taaatatttg
     9361 tatgtacaat ttatttgctc caatatattt gtatatattt ccctcacagc tatatttatt
     9421 ctaatttaat attatgactt tttaaggtaa ttttttgtga cctgttcgga gtgattagcg
     9481 ttacaatttg aactgaaagt gacatccagt gtttgttcct tgtgtagatg catctcaaaa
     9541 aaatggtggg cataatagtg ttgtttatat atatcaaaaa taacaactat aataataaga
     9601 atacatttaa tttagaaaat gcttggattt cactggaact agaattaatt cggctgctgc
     9661 tctaaacgac gcatttcgta ctccaaagta cgaatttttt ccctcaagct cttattttca
     9721 ttaaacaatg aacaggacct aacgcacagt cacgttattg tttacataaa tgattttttt
     9781 tactattcaa acttactctg tttgtgtact cccactggta tagccttctt ttatcttttc
     9841 tggttcaggc tctatcactt tactaggtac ggcatctgcg ttgagtcgcc tccttttaaa
     9901 tgtctgacct tttgcaggtg cagccttcca ctgcgaatca ttaaagtggg tatcacaaat
     9961 ttgggagttt tcaccaaggc tgcacccaag gctctgctcc cacaattttc tcttaatagc
    10021 acacttcggc acgtgaatta attttactcc agtcacagct ttgcagcaaa atttgcaata
    10081 tttcattttt ttttattcca cgtaagggtt aatgttttca aaaaaaaatt cgtccgcaca
    10141 caacctttcc tctcaacaag caaacgtgca ctgaatttaa gtgtatactt cggtaagctt
    10201 cggctatcga cgggaccacc ttatgttatt tcatcatg
//
