LOCUS       sequence-11395-e       10380 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10380)
  AUTHORS   Potter CJ, Tasic B, Russler EV, Liang L, Luo L
  TITLE     The Q system: a repressible binary system for transgene expression, 
            lineage tracing, and mosaic analysis.
  JOURNAL   Cell. 2010 Apr 30. 141(3):536-48.
  PUBMED    20434990
REFERENCE   2  (bases 1 to 10380)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 30. 141(3):536-48."
COMMENT     Sequence Label: pQF-M2ET
FEATURES             Location/Qualifiers
     source          1..10380
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          3368..3433
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3563..3583
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3855..3989
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3892..3911)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3946..3965
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4016..4034)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(4494..4565,4960..5233,5308..5962,6024..6339,6560..6691,
                     6762..7376)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    8161..8194
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        8220..8324
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8325..8393,8394..9185)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMSVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8543..8562)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9356..9944
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9845..9864
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    join(10146..10347,10348..10378)
                     /label=P element 3' end
                     /note="P element 3' end"
ORIGIN
        1 cgttcccatg tgttggtctg tatgccgtag tgctgccgtc gcagcggttt tcacgccaaa
       61 tctggcttgt ttcagaggtc aggaactaac ttagtctgca ggtgtagtta gcatgttccc
      121 gcctacacag gttcactgaa caaaagtatt taaacataaa aatatctatt ttatagatac
      181 aatttttctt gttcttatag tttttactaa gaaaacgatt agttataaaa aatattatgt
      241 aagtgatgtc aaaaagaaag tagcatgtcg ttggtattct tttcataatt gagttcaagg
      301 ctaaactttt tctattccat atatttttaa ataatttata tcattacccg aattatattt
      361 caaaggaaaa acagttaggt aaactttctt ctattatatt tttactcttt aaaaactttc
      421 ctatgcattg tagtaacgtt ttagattttt ttttgcctat ttatttaaac ataaatcagc
      481 aaattctatt ttattcataa atgtcccacc aaccaatgtt cttcaagaca atagcctaca
      541 gcactagtgc cccagttcgg gcaaggtcat cctggagacg cttaacttct ccgccgccga
      601 tctgccgctg gactacgtgg gtctggccca tgatgaaata acataaggtg gtcccgtcga
      661 tagccgaagc ttaccgaagt atacacttaa attcagtgca cgtttgcttg ttgagaggaa
      721 aggttgtgtg cggacgaatt tttttttgaa aacattaacc cttacgtgcg gccgcggata
      781 acttcgtata agaaaccata tacgaagtta tctcgagaat tccaacatgc cgcctaaacg
      841 caagacactc aatgccgctg ccgaagccaa tgcccacgct gatggccatg ctgatggcaa
      901 tgctgatggt catgtcgcta acactgcagc aagcagcaac aacgcccgtt ttgcggactt
      961 gaccaacatt gacacacccg gcctcggccc taccacgacg acgttacttg tcgagcccgc
     1021 tcgttcgaaa cgccagagag tctcgagggc ctgtgatcag tgtcgagctg cacgtgaaaa
     1081 gtgtgatgga atccagccgg cttgcttccc ctgtgtgtcg cagggccggt cgtgtaccta
     1141 ccaggccagt cccaagaagc gaggagtcca gacgggctac atccgcactc tcgaactggc
     1201 tctggcttgg atgttcgaga acgttgcccg cagcgaggac gccctccaca atcttttggt
     1261 ccgtgatgct ggccagggca gcgctctcct ggtcggcaaa gactcgcctg ctgcagaacg
     1321 cctgcatgca agatgggcga cgagtcgagt caacaaaagc atcacccgtc ttctctcagg
     1381 tcaggccgca caagatccat ctgaagacgg ccaatccccg tccgaagaca taaatgtcca
     1441 agatgccggg gcaaagacat ccgacttccc tcatgcgcct cacttgactt tctcggcgcc
     1501 taagtctagt acagctgaga cacgcactct accaggcccg gtccgaccac ctatttcggc
     1561 aaacaccctg gaaaacaacc ttcagccaga tggtaccggg atagggaagc taccacccaa
     1621 tcattggcgc ctgctggata tctacttttc ctacacgcat tcttggctcc ctatcctcga
     1681 gaagaaagac atgtaccaag cattgtacca gtactctgaa caaggctcgt tgcttccctc
     1741 tgcgaatgtc gagtctggcg ttcatgccga gctctggagc gcgctcgccc tggcgtcctt
     1801 ccaggctgct gctactgctg catcgagtgc tacgggtcca gcttcagctg ctcatggcca
     1861 tgacaatgcc atcaatcctt cacctgcaga catatctgac acagcccgaa agctcatacc
     1921 tttggaaagc gggccgttcc aggttcagca ctgcagagcg ttgctgcttc tttgtctcgt
     1981 aagccttggg cgggatgatt gggagtctgc ttggttgctg gttggctttg cggtccgcgt
     2041 cctacttgtt gttcgcaccc agttgcctcc tgatgatgac cggccacgac caagaatgcg
     2101 tgcgctgctc gtcgcgtgct tcatcgtgga taccattgtg tctatgagac acaacgtgcc
     2161 ggcccatctc aagccagacg acattgcgga tctgccgtta cctgaagacg gtcaagatca
     2221 atgggagccg tggacaccat gtgagggctt aggcggtgaa cacaccatgc tgcaaatgtt
     2281 gaggaacccg gcataccctt taagcacatt caaccaccta tatggcgtga ccaagctggt
     2341 tgctttggag cttctgccaa gaatacgaac atcttcacag aacgctccct tggagttcag
     2401 gtcgcggttg cagcaggtaa tcggccacaa ttctcccttc agcgtctttg tcctttccca
     2461 ggatacagca tcggcttttg tgcctactgc ataccttacc cgtaccgttt atttatgggc
     2521 agctgccttt tctgagcctc tcaacgaaca ctactcgcat cttctgatcg aaactcttga
     2581 tcagtatcag aagcggtttg gtacatatgc aatcccacct ctgatccctt ctcttctaga
     2641 ctcccttctt gctttaaaga aacaatcaca ttcttcagag cggcatcgaa ggcacttgga
     2701 agagcttttc cccgcctact cctccatttg gcctcgggga ggccgacaca gcaatactgg
     2761 cctccaaccc atacgacaac ttgagcttcc gccgactgcg actgccactg caagtatcat
     2821 gccccatgtc atggaacagc ccctgtcaac gtcaataaat ccggtcaatg atcggtttaa
     2881 tggaataccg aatcctaccc cctacaatag cgatgcggcc ctagacgcaa ttactcaaac
     2941 caatgactac ggatcagtca atacccatgg cattctcagt acatatccac cacctgccac
     3001 ccacttgaac gaggcctcgg tggcccttgc gcctggaggt gctcctccca gaccaccccc
     3061 gccatacgtt gatagtacaa cgaaccatcc tccttaccat agtaacctcg tccccatggc
     3121 caatttcgga tattcgactg tagactatga tgcgatggtg gatgatctgg catcgatcga
     3181 gtacacggat gcggttgacg ttgatccgca gttcatgacg aacctcgggt ttgtcccagg
     3241 gtgtaatttt agcgatatca acacgtatgg caataggcgg ccgctctaga ggatctttgt
     3301 gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag
     3361 ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt
     3421 ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt
     3481 taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc
     3541 tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt
     3601 tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg
     3661 ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa
     3721 atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg
     3781 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     3841 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     3901 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     3961 tcatcaatgt atcttatcat gtctggatcg gatccactag aaggccttag tatgtatgta
     4021 agttaataaa accctttttt ggagaatgta gatttaaaaa aacatatttt ttttttattt
     4081 tttactgcac tggacatcat tgaacttatc tgatcagttt taaatttact tcgatccaag
     4141 ggtatttgaa gtaccaggtt ctttcgatta cctctcactc aaaatgacat tccactcaaa
     4201 gtcagcgctg tttgcctcct tctctgtcca cagaaatatc gccgtctctt tcgccgctgc
     4261 gtccgctatc tctttcgcca ccgtttgtag cgttacctag cgtcaatgtc cgccttcagt
     4321 tgcactttgt cagcggtttc gtgacgaagc tccaagcggt ttacgccatc aattaaacac
     4381 aaagtgctgt gccaaaactc ctctcgcttc ttatttttgt ttgttttttg agtgattggg
     4441 gtggtgattg gttttgggtg ggtaagcagg ggaaagtgtg aaaaatcccg gcaatgggcc
     4501 aagaggatca ggagctatta attcgcggag gcagcaaaca cccatctgcc gagcatctga
     4561 acaatgtgag tagtacatgt gcatacatct taagttcact tgatctatag gaactgcgat
     4621 tgcaacatca aattgtctgc ggcgtgagaa ctgcgaccca caaaaatccc aaaccgcaat
     4681 cgcacaaaca aatagtgaca cgaaacagat tattctggta gctgtgctcg ctatataaga
     4741 caatttttaa gatcatatca tgatcaagac atctaaaggc attcattttc gactacattc
     4801 ttttttacaa aaaatataac aaccagatat tttaagctga tcctagatgc acaaaaaata
     4861 aataaaagta taaacctact tcgtaggata cttcgttttg ttcggggtta gatgagcata
     4921 acgcttgtag ttgatatttg agatccccta tcattgcagg gtgacagcgg agcggcttcg
     4981 cagagctgca ttaaccaggg cttcgggcag gccaaaaact acggcacgct cctgccaccc
     5041 agtccgccgg aggactccgg ttcagggagc ggccaactag ccgagaacct cacctatgcc
     5101 tggcacaata tggacatctt tggggcggtc aatcagccgg gctccggatg gcggcagctg
     5161 gtcaaccgga cacgcggact attctgcaac gagcgacaca taccggcgcc caggaaacat
     5221 ttgctcaaga acggtgagtt tctattcgca gtcggctgat ctgtgtgaaa tcttaataaa
     5281 gggtccaatt accaatttga aactcagttt gcggcgtggc ctatccgggc gaacttttgg
     5341 ccgtgatggg cagttccggt gccggaaaga cgaccctgct gaatgccctt gcctttcgat
     5401 cgccgcaggg catccaagta tcgccatccg ggatgcgact gctcaatggc caacctgtgg
     5461 acgccaagga gatgcaggcc aggtgcgcct atgtccagca ggatgacctc tttatcggct
     5521 ccctaacggc cagggaacac ctgattttcc aggccatggt gcggatgcca cgacatctga
     5581 cctatcggca gcgagtggcc cgcgtggatc aggtgatcca ggagctttcg ctcagcaaat
     5641 gtcagcacac gatcatcggt gtgcccggca gggtgaaagg tctgtccggc ggagaaagga
     5701 agcgtctggc attcgcctcc gaggcactaa ccgatccgcc gcttctgatc tgcgatgagc
     5761 ccacctccgg actggactca tttaccgccc acagcgtcgt ccaggtgctg aagaagctgt
     5821 cgcagaaggg caagaccgtc atcctgacca ttcatcagcc gtcttccgag ctgtttgagc
     5881 tctttgacaa gatccttctg atggccgagg gcagggtagc tttcttgggc actcccagcg
     5941 aagccgtcga cttcttttcc tagtgagttc gatgtgttta ttaagggtat ctagcattac
     6001 attacatctc aactcctatc cagcgtgggt gcccagtgtc ctaccaacta caatccggcg
     6061 gacttttacg tacaggtgtt ggccgttgtg cccggacggg agatcgagtc ccgtgatcgg
     6121 atcgccaaga tatgcgacaa ttttgctatt agcaaagtag cccgggatat ggagcagttg
     6181 ttggccacca aaaatttgga gaagccactg gagcagccgg agaatgggta cacctacaag
     6241 gccacctggt tcatgcagtt ccgggcggtc ctgtggcgat cctggctgtc ggtgctcaag
     6301 gaaccactcc tcgtaaaagt gcgacttatt cagacaacgg tgagtggttc cagtggaaac
     6361 aaatgatata acgcttacaa ttcttggaaa caaattcgct agattttagt tagaattgcc
     6421 tgattccaca cccttcttag tttttttcaa tgagatgtat agtttatagt tttgcagaaa
     6481 ataaataaat ttcatttaac tcgcgaacat gttgaagata tgaatattaa tgagatgcga
     6541 gtaacatttt aatttgcaga tggttgccat cttgattggc ctcatctttt tgggccaaca
     6601 actcacgcaa gtgggcgtga tgaatatcaa cggagccatc ttcctcttcc tgaccaacat
     6661 gacctttcaa aacgtctttg ccacgataaa tgtaagtctt gtttagaata catttgcata
     6721 ttaataattt actaactttc taatgaatcg attcgattta ggtgttcacc tcagagctgc
     6781 cagtttttat gagggaggcc cgaagtcgac tttatcgctg tgacacatac tttctgggca
     6841 aaacgattgc cgaattaccg ctttttctca cagtgccact ggtcttcacg gcgattgcct
     6901 atccgatgat cggactgcgg gccggagtgc tgcacttctt caactgcctg gcgctggtca
     6961 ctctggtggc caatgtgtca acgtccttcg gatatctaat atcctgcgcc agctcctcga
     7021 cctcgatggc gctgtctgtg ggtccgccgg ttatcatacc attcctgctc tttggcggct
     7081 tcttcttgaa ctcgggctcg gtgccagtat acctcaaatg gttgtcgtac ctctcatggt
     7141 tccgttacgc caacgagggt ctgctgatta accaatgggc ggacgtggag ccgggcgaaa
     7201 ttagctgcac atcgtcgaac accacgtgcc ccagttcggg caaggtcatc ctggagacgc
     7261 ttaacttctc cgccgccgat ctgccgctgg actacgtggg tctggccatt ctcatcgtga
     7321 gcttccgggt gctcgcatat ctggctctaa gacttcgggc ccgacgcaag gagtagccga
     7381 catatatccg aaataactgc ttgttttttt ttttaccatt attaccatcg tgtttactgt
     7441 ttattgcccc ctcaaaaagc taatgtaatt atatttgtgc caataaaaac aagatatgac
     7501 ctatagaata caagtatttc cccttcgaac atccccacaa gtagactttg gatttgtctt
     7561 ctaaccaaaa gacttacaca cctgcatacc ttacatcaaa aactcgttta tcgctacata
     7621 aaacaccggg atatattttt tatatacata cttttcaaat cgcgcgccct cttcataatt
     7681 cacctccacc acaccacgtt tcgtagttgc tctttcgctg tctcccaccc gctctccgca
     7741 acacattcac cttttgttcg acgaccttgg agcgactgtc gttagttccg cgcgattcgg
     7801 ttcgctcaaa tggttccgag tggttcattt cgtctcaata gaaattagta ataaatattt
     7861 gtatgtacaa tttatttgct ccaatatatt tgtatatatt tccctcacag ctatatttat
     7921 tctaatttaa tattatgact ttttaaggta attttttgtg acctgttcgg agtgattagc
     7981 gttacaattt gaactgaaag tgacatccag tgtttgttcc ttgtgtagat gcatctcaaa
     8041 aaaatggtgg gcataatagt gttgtttata tatatcaaaa ataacaacta taataataag
     8101 aatacattta atttagaaaa tgcttggatt tcactggaac tagaattaat tccaaacgtc
     8161 ataacttcgt ataatgtatg ctatacgaag ttataggtgg cacttttcgg ggaaatgtgc
     8221 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     8281 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     8341 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     8401 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     8461 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     8521 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc
     8581 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     8641 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     8701 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     8761 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     8821 agctgaatga agccatacca aacgacgagc gtgacaccac gatgtctgta gcaatggcaa
     8881 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     8941 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     9001 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     9061 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     9121 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     9181 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     9241 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     9301 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     9361 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     9421 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     9481 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     9541 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     9601 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     9661 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     9721 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     9781 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     9841 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     9901 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcaa
     9961 gcttctagag atctgcaggt cgacggatcc gtcgagcgta taaccatctg tacaaaaaag
    10021 gatttccttt gcccagtcgt acgactttgt acagatggtt atcagatgtg gacataaaaa
    10081 gaggatgttt ggatgtggtc atagacctaa tggacagtga tggagttgat gacgccgaca
    10141 agctttgcgt actcgcaaat tattaaaaat aaaactttaa aaataatttc gtctaattaa
    10201 tattatgagt taattcaaac cccacggaca tgctaagggt taatcaacaa tcatatcgct
    10261 gtctcactca gactcaatac gacactcaga atactattcc tttcactcgc acttattgca
    10321 agcatacgtt aagtggatgt ctcttgccga cgggaccacc ttatgttatt tcatcatggg
//
