LOCUS       sequence-113999-         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses gRNA against human GABPA along with Cas9 with 2A GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts 
            BS, Mackiewicz M, Mendenhall EM, Myers RM
  TITLE     CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins.
  JOURNAL   Genome Res. 2015 Sep 9.
  PUBMED    26355004
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2015 Sep 9."
COMMENT     Sequence Label: PX458_GABPA_2
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      40..49
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             49..114
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             121..141
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gcacctgcct gaaatcactt tttttcaggt tggaccggtg ccaccatgga ctataaggac
       61 cacgacggag actacaagga tcatgatatt gattacaaag acgatgacga taagatggcc
      121 ccaaagaaga agcggaaggt cggtatccac ggagtcccag cagccgacaa gaagtacagc
      181 atcggcctgg acatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag
      241 gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac
      301 ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg gctgaagaga
      361 accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc
      421 agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg
      481 gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg
      541 gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc
      601 gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc
      661 cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc
      721 cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg
      781 gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga aaatctgatc
      841 gcccagctgc ccggcgagaa
//
