LOCUS       sequence-114017-         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Chloride-conducting Channelrhodopsin with neuron-specific promoter, 
            plus red fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Wietek J, Beltramo R, Scanziani M, Hegemann P, Oertner TG, Simon 
            Wiegert J
  TITLE     An improved chloride-conducting channelrhodopsin for light-induced 
            inhibition of neuronal activity in vivo.
  JOURNAL   Sci Rep. 2015 Oct 7;5:14807. doi: 10.1038/srep14807.
  PUBMED    26443033
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-10-7"; volume: "5:14807. doi"; pages: " 
            10.1038/srep14807"
COMMENT     Sequence Label: pAAV CaMKIIa iChloC 2A tDimer
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..927
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEQIYVCAIRMVKVILEFFFSFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVILIHLSNLTGLSNDYSRRTMGLLVSNIGCIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
ORIGIN
        1 atggattatg gaggcgccct gagtgccgtt gggcgcgagc tgctatttgt aacgaaccca
       61 gtagtcgtca atggctctgt acttgtgcct gaggaccagt gttactgcgc gggctggatt
      121 gagtcgcgtg gcacaaacgg tgcccaaacg gcgtcgaacg tgctgcaatg gcttgctgct
      181 ggcttctcca tcctactgct tatgttttac gcctaccaaa catggaagtc aacctgcggc
      241 tgggagcaga tctatgtgtg cgctatcagg atggtcaagg tgattctcga gttcttcttc
      301 tcgtttaaga acccgtccat gctgtatcta gccacaggcc accgcgtcca gtggttgcgt
      361 tacgccgagt ggcttctcac ctgcccggtc attctcattc acctgtcaaa cctgacgggc
      421 ttgtccaacg actacagcag gcgcaccatg ggtctgcttg tgtctaatat tggctgtatt
      481 gtgtggggcg ccacttccgc catggccacc ggatacgtca aggtcatctt cttctgcctg
      541 ggtctgtgtt atggtgctaa cacgttcttt cacgctgcca aggcctacat cgagggttac
      601 cacaccgtgc cgaagggccg gtgtcgccag gtggtgactg gcatggcttg gctcttcttc
      661 gtatcatggg gtatgttccc catcctgttc atcctcggcc ccgagggctt cggcgtcctg
      721 agcgtgtacg gctccaccgt cggccacacc atcattgacc tgatgtcgaa gaactgctgg
      781 ggtctgctcg gccactacct gcgcgtgctg atccacgagc atatcctcat ccacggcgac
      841 attcgcaaga ccaccaaatt gaacattggt ggcactgaga ttgaggtcga gacgctggtg
      901 gaggacgagg ccgaggctgg cgcggta
//
