LOCUS       sequence-114140-         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoClo Basic Part: Controllable promoter - pBAD - araC regulated 
            (pBAD promoter, inducible by expression of AraC, C0080) 
            [F:I13453:B].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Iverson SV, Haddock TL, Beal J, Densmore DM
  TITLE     CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part 
            Library Enable Rapid Combinatorial Design for Synthetic and 
            Traditional Biology.
  JOURNAL   ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 
            10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
  PUBMED    26479688
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00124"; journalName: "ACS Synth Biol"; date: 
            "2016-01-15- 15"; volume: "5"; issue: "1"; pages: "99-103"
COMMENT     Sequence Label: I13453 (pBAD)_FB
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(22..65)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    68..89
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     primer_bind     141..160
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     misc_feature    249..269
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(275..292)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(526..545)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg
       61 atttctggaa ttcgcggccg cttctagaga ctagtgggtc tcacgctaca ttgattattt
      121 gcacggcgtc acactttgct atgccatagc atttttatcc ataagattag cggatcctac
      181 ctgacgcttt ttatcgcaac tctctactgt ttctccatac cgtttttttg ggctagctac
      241 tagagaccta ctagtagcgg ccgctgcagg cttcctcgct cactgactcg ctgcgctcgg
      301 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
      361 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
      421 gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg cccccctgac gagcatcaca
      481 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      541 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      601 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
      661 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      721 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      781 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
      841 ctacagagtt cttgaagtgg tggcctaact acggctacac tag
//
