LOCUS       sequence-114151-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoClo Basic Part: RBS - Weiss RBS, high strength. Modified from 
            Bba_B0034 to adjust spacing in MC system. [B:B0034m:C].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Iverson SV, Haddock TL, Beal J, Densmore DM
  TITLE     CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part 
            Library Enable Rapid Combinatorial Design for Synthetic and 
            Traditional Biology.
  JOURNAL   ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 
            10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
  PUBMED    26479688
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00124"; journalName: "ACS Synth Biol"; date: 
            "2016-01-15- 15"; volume: "5"; issue: "1"; pages: "99-103"
COMMENT     Sequence Label: B0034m_BC
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(22..65)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    68..89
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     RBS             112..123
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    140..160
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(166..183)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(417..436)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg
       61 atttctggaa ttcgcggccg cttctagaga ctagtgggtc tcatactaga gaaagaggag
      121 aaatactaaa tgagagacct actagtagcg gccgctgcag gcttcctcgc tcactgactc
      181 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
      241 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
      301 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cacaggctcc gcccccctga
      361 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
      421 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
      481 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
      541 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
      601 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
      661 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
      721 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
      781 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaa
//
