LOCUS       sequence-114161-         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli vector for expression of S. pyogenes dCas9, tracrRNA, and 
            nontargeting CRISPR array with BsaI site for inserting user-defined 
            spacer-repeat bricks.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Cress BF, Toparlak OD, Guleria S, Lebovich M, Stieglitz JT, 
            Englaender JA, Jones JA, Linhardt RJ, Koffas MA
  TITLE     CRISPathBrick: Modular Combinatorial Assembly of Type II-A CRISPR 
            Arrays for dCas9-Mediated Multiplex Transcriptional Repression in E.
            coli.
  JOURNAL   ACS Synth Biol. 2015 Mar 30.
  PUBMED    25822415
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Mar 30."
COMMENT     Sequence Label: pCRISPathBrick
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   388..423
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   454..489
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     misc_feature    complement(490..621)
                     /label=crRNA leader
                     /note="crRNA leader sequence for the Streptococcus pyogenes
                     CRISPR/Cas system"
ORIGIN
        1 atgcgtccgg cgtagaggat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata
       61 gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct
      121 ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag
      181 tgactggcga tgctgtcgga atggacgatc acactactct tcttttgcct attataacat
      241 gaaaacgctt taacagaaag aataggaagg tatccgactg ctggtattaa ccctctttct
      301 caagttatca tcggcaatgt tgaatggagt ccattcaaaa cagcatagct ctaaaacatg
      361 gaactcaaca agtctcagtg tgctgaagtt ttgggaccat tcaaaacagc atagctctaa
      421 aacgagtcct atgagcttcc gagacaggtc tcagttttgg gaccattcaa aacagcatag
      481 ctctaaaacc tcgtagacta tttttgtcta aaaaatttcg taatcgcact atttgtctca
      541 gctagacttc agtcttgaaa agcccctgta ttactgcatt tattaagagt attataccat
      601 atttttagtt attaagaaat aatcttcatc taaaatatac ttcagtcacc tcctagctga
      661 ctcaaatcaa tgcgtgtttc ataaagacca gtgatggatt gatggataag agtggcatct
      721 aaaacttctt ttgtagacgt atatcgttta cgatcaattg ttgtatcaaa atatttaaaa
      781 gcagcgggag ctccaagatt cgtcaacgta aataaatgaa taatattttc tgcttgttca
      841 cgtattggtt tgtct
//
