LOCUS       sequence-114171-         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoClo Basic Part: CDS - Controller protein, tetR repressor 
            (represses pTet, C0040. can be inhibited by tetracyclin or aTc) 
            [C:C0040:D].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Iverson SV, Haddock TL, Beal J, Densmore DM
  TITLE     CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part 
            Library Enable Rapid Combinatorial Design for Synthetic and 
            Traditional Biology.
  JOURNAL   ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 
            10.1021/acssynbio.5b00124. Epub 2015 Nov 4.
  PUBMED    26479688
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00124"; journalName: "ACS Synth Biol"; date: 
            "2016-01-15- 15"; volume: "5"; issue: "1"; pages: "99-103"
COMMENT     Sequence Label: C0040 (tetR)_CD
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(22..65)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    68..89
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             105..725
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(178..197)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             726..758
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag (LVA)
                     /note="mutant LVA variant that confers accelerated 
                     degradation under some conditions (Andersen et al., 1998)"
                     /translation="AANDENYALVA"
     misc_feature    776..796
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(802..819)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg
       61 atttctggaa ttcgcggccg cttctagaga ctagtgggtc tcaaatgtcc agattagata
      121 aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc gaaggtttaa
      181 caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat tggcatgtaa
      241 aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg caccatactc
      301 acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac gctaaaagtt
      361 ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt acacggccta
      421 cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa caaggttttt
      481 cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta ggttgcgtat
      541 tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact actgatagta
      601 tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca gagccagcct
      661 tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg
      721 ggtccgctgc aaacgacgaa aactacgctt tagtagctta ataaaggtag agacctacta
      781 gtagcggccg ctgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
      841 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat cagg
//
