LOCUS       sequence-114198-         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This a retroviral expression plasmid expressing GFP tagged G12C 
            mutant Kras 2B oncogene along with hygro resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Agalioti T, Giannou AD, Krontira AC, Kanellakis NI, Kati D, Vreka M,
            Pepe M, Spella M, Lilis I, Zazara DE, Nikolouli E, Spiropoulou N, 
            Papadakis A, Papadia K, Voulgaridis A, Harokopos V, Stamou P, 
            Meiners S, Eickelberg O, Snyder LA, Antimisiaris SG, Kardamakis D, 
            Psallidas I, Marazioti A, Stathopoulos GT
  TITLE     Mutant KRAS promotes malignant pleural effusion formation.
  JOURNAL   Nat Commun. 2017 May 16;8:15205. doi: 10.1038/ncomms15205.
  PUBMED    28508873
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-05-16"; volume: "8:15205. doi"; pages: " 
            10.1038/ncomms15205"
COMMENT     Sequence Label: 8798 GFP-KrasG12C_2B_hygro
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gctggggcgt cggtttccac tatcggcgag tacttctaca cagccatcgg tccagacggc
       61 cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg gctccggatc ggacgattgc
      121 gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca agctctgata
      181 gagttggtca agaccaatgc ggagcatata cgcccggagc cgcggcgatc ctgcaagctc
      241 cggatgcctc cgctcgaagt agcgcgtctg ctgctccata caagccaacc acggcctcca
      301 gaagaagatg ttggcgacct cgtattggga atccccgaac atcgcctcgc tccagtcaat
      361 gaccgctgtt atgcggccat tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac
      421 gaggtgccgg acttcggggc agtcctcggc ccaaagcatc agctcatcga gagcctgcgc
      481 gacggacgca ctgacggtgt cgtccatcac agtttgccag tgatacacat ggggatcagc
      541 aatcgcgcat atgaaatcac gccatgtagt gtattgaccg attccttgcg gtccgaatgg
      601 gccgaacccg ctcgtctggc taagatcggc cgcagcgatc gcatccatgg cctccgcgac
      661 cggctgcaga acagcgggca gttcggtttc aggcaggtct tgcaacgtga caccctgtgc
      721 acggcgggag atgcaatagg tcaggctctc gctgaattcc ccaatgtcaa gcacttccgg
      781 aatcgggagc gcggccgatg caaagtgccg ataaacataa cgatctttgt agaaaccatc
      841 ggcgcagcta tttacccgca ggacatatcc acgccctcct acatcgaagc tgaaagcacg
      901 agattcttcg ccct
//
