LOCUS       sequence-114235-         672 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes RVD positions 4-6 in TALEN assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Ma AC, McNulty MS, Tanya PL, Jarryd CM, Martinez-Galvez G, Argue DP,
            Lee HB, Urban MD, Bullard CE, Blackburn PR, Man TK, Clark KJ, Ekker 
            SC
  TITLE     FusX: A rapid one-step TALE assembly system for genome science.
  JOURNAL   Hum Gene Ther. 2016 Jun;27(6):451-63. doi: 10.1089/hum.2015.172
  PUBMED    26854857
REFERENCE   2  (bases 1 to 672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1089/hum.2015"; 
            journalName: "Hum Gene Ther"; date: "2016-06"; volume: "27"; issue: 
            "6"; pages: "451-63"
COMMENT     Sequence Label: pFusX2-22
FEATURES             Location/Qualifiers
     source          1..672
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(386..403)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(386..402)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(395..417)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      466..552
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      644..671
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gtcacccggc aaccttgggt agcagcgaag tcgaggcatt tctgtcctgg ctggtctaga
       61 cgtctcgcca tggtcctggc acagcaccgg caacagccgc tgcaccgttt cgagcgcttg
      121 cttgccgcca tcgtggctgg cgatagccac cacttggtcc ggagtcaggc catggtcctg
      181 gcacagcacc ggcaacagcc gctgcaccgt ttcgagcgct tgcttgccgc catcgtggct
      241 ggcgatagcc accacttggt ccggagtcag gccatggtcc tggcacagca ccggcaacag
      301 ccgctgcacc gtttcgagcg cttgcttgcc gccatcgtgg ctggcgatag ccaccacttg
      361 gtccggggtc agggagacgc ttaagactgg ccgtcgtttt acaacgtcgt gactgggaaa
      421 acatccatgc tagcgttaac gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag
      481 gctcagtcgg aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
      541 agtaggacaa atccgccggg agcggatttg aacgttgtga agcaacggcc cggagggtgg
      601 cgggcaggac gcccgccata aactgccagg catcaaacta agcagaaggc catcctgacg
      661 gatggccttt tt
//
