LOCUS       sequence-114309-         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral vector expressing mouse TRF1 lacking BLM-binding motifs 
            with N-terminal Myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Zimmermann M, Kibe T, Kabir S, de Lange T
  TITLE     TRF1 negotiates TTAGGG repeat-associated replication problems by 
            recruiting the BLM helicase and the TPP1/POT1 repressor of ATR 
            signaling.
  JOURNAL   Genes Dev. 2014 Nov 15;28(22):2477-91. doi: 10.1101/gad.251611.114. 
            Epub 2014 Oct 24.
  PUBMED    25344324
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.251611.114"; journalName: "Genes Dev"; date: 
            "2014-11-15- 15"; volume: "28"; issue: "22"; pages: "2477-91"
COMMENT     Sequence Label: pLPC-NMYC-mTRF1deltaBLM
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..47
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        28..46
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      78..87
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             87..116
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 aaccactgct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagcttg
       61 gtaccgagct cggatcagcc accatggaac aaaagttgat ttctgaagaa gatttgttaa
      121 gaaagagagg atccgcggag acggtctcct cagcggcccg ggacgcgccg agccgtgagg
      181 gctggacaga ttcggattct ccagagcagg aggaggtggg agacgacgcg gagctgctcc
      241 agtgccagct tcagctgggg accccgagag agatggagaa cgcggagctt gtggctgagg
      301 tggaggccgt ggctgcgggc tggatgctcg acttcctctg cctgtctctg tgccgagcct
      361 tccgcgacgg ccgctccgag gactttcgtc gtactcgtga cagcgccgag gctattattc
      421 atggactaca cagacttaca gcttaccaat tgaaaactgt gtatatatgt cagtttttga
      481 caagagttgc atctggaaag gcccttgatg cacagtttga agttgatgag cgtattacac
      541 ccttggaatc agccctgatg atttggaact caattgaaaa ggaacatgac aaactgcatg
      601 acgaaataaa gaatttaatt aaaattcagg ctgtagctgt ttgtatggaa attggcagct
      661 ttaaggaagc agaagaagta tttgaaagaa tatttggtga tccagaattt tacacgcctt
      721 tagaaaggaa gttacttaag ataatctctc agaaggatgt gttccactcc cttttccaac
      781 acttcagcta tagctgcatg atggagaaaa ttcagagtta tgtgggtgat gtgttaagtg
      841 aaaaatcatc aacttttcta atgaaggcag caacaaaagt agtggaaaa
//
