LOCUS       sequence-114329-         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral vector expressing human TRF1 with N-terminal MYC tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Zimmermann M, Kibe T, Kabir S, de Lange T
  TITLE     TRF1 negotiates TTAGGG repeat-associated replication problems by 
            recruiting the BLM helicase and the TPP1/POT1 repressor of ATR 
            signaling.
  JOURNAL   Genes Dev. 2014 Nov 15;28(22):2477-91. doi: 10.1101/gad.251611.114. 
            Epub 2014 Oct 24.
  PUBMED    25344324
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.251611.114"; journalName: "Genes Dev"; date: 
            "2014-11-15- 15"; volume: "28"; issue: "22"; pages: "2477-91"
COMMENT     Sequence Label: pLPC-MYC-hTRF1
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     26..45
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        26..44
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      76..85
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             85..114
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagcttggt
       61 accgagctcg gatcagccac catggaacaa aagttgattt ctgaagaaga tttgttaaga
      121 aagagaggat ccgcggagga tgtttcctca gcggccccga gcccgcgggg ctgtgcggat
      181 ggtagggatg ccgaccctac tgaggagcag atggcagaaa cagagagaaa cgacgaggag
      241 cagttcgaat gccaggaact gctcgagtgc caggtgcagg tgggggcccc cgaggaggag
      301 gaggaggagg aggaggacgc gggcctggtg gccgaggccg aggccgtggc tgccggctgg
      361 atgctcgatt tcctctgcct ctctctttgc cgagctttcc gcgacggccg ctccgaggac
      421 ttccgcagga cccgcaacag cgcagaggct attattcatg gactatccag tctaacagct
      481 tgccagttga gaacgatata catatgtcag tttttgacaa gaattgcagc aggaaaaacc
      541 cttgatgcac agtttgaaaa tgatgaacga attacaccct tggaatcagc cctgatgatt
      601 tggggttcaa ttgaaaagga acatgacaaa cttcatgaag aaatacagaa tttaattaaa
      661 attcaggcta tagctgtttg tatggaaaat ggcaacttta aagaagcaga agaagtcttt
      721 gaaagaatat ttggtgatcc aaattctcat atgcctttca aaagcaaatt gcttatgata
      781 atctctcaga aagatacatt tcattccttt tttcaacact tcagctacaa ccacatgatg
      841 gagaaaatta agagttatgt gaattatgtg ctaagtgaaa aatcatcaac cttt
//
