LOCUS       sequence-114372-         671 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HBV polymerase in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Ryu DK, Kim S, Ryu WS
  TITLE     Hepatitis B virus polymerase suppresses translation of pregenomic 
            RNA via a mechanism involving its interaction with 5' stem-loop 
            structure.
  JOURNAL   Virology. 2008 Mar 30;373(1):112-23. Epub 2007 Dec 21.
  PUBMED    18155120
REFERENCE   2  (bases 1 to 671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virology.";
            date: "2008-03-30"; volume: "373(1)"; pages: "112-23. Epub 2007 Dec 
            21"
COMMENT     Sequence Label: Pol
FEATURES             Location/Qualifiers
     source          1..671
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     25..44
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        25..43
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             63..128
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc aagcttacca
       61 tggactacaa agaccatgac ggtgattata aagatcatga catcgactac aaggatgacg
      121 atgacaagat gcccctatcc tatcaacact tccggagact actgttgtta gacgacgagg
      181 caggtcccct agaagaagaa ctccctcgcc tcgcagacga aggtctcaat cgccgcgtcg
      241 cagaagatct caatctcggg aatctcaatg ttagtattcc ttggactcat aaggtgggga
      301 actttactgg gctttattct tctactgtac ctgtctttaa tcctcattgg aagacaccat
      361 cttttcctaa tatacattta caccaagaca ttatcaaaaa atgtgaacag tttgtaggcc
      421 cactcacagt taatgagaaa agaagattgc aattgatcat gcctgccagg ttttatccaa
      481 aggttaccaa atatttacca ttggataagg gtattaaacc ttattatcca gaacatctag
      541 ttaatcatta cttccaaact agacactatt tacacactct atggaaggcg ggtatattat
      601 ataagagaga aacgacacat agcgcctcat tttgtgggtc accgtattct tgggaacaag
      661 atctacagca t
//
